Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 9774562Journal NLM ID: 7505564Publisher: American Society for Microbiology
Correspondence: reeves
angis.su.oz.au
Institutions: Department of Microbiology, The University of Sydney, New South Wales 2006, Australia
Shiga toxin (Stx)-producing Escherichia coli strains of serogroup O111 are the most frequently isolated non-O157 strains causing outbreaks of gastroenteritis with hemolytic-uremic syndrome. The O111 O-antigen gene cluster had been cloned and about half of it has been sequenced; we have now sequenced the remainder of the gene cluster, which is 12.5 kb in length and which comprises 11 genes. On the basis of sequence similarity, we have identified all the O-antigen genes expected, including five sugar biosynthetic pathway genes, three transferase genes, the O-unit flippase gene, and the O-antigen polymerase gene. By PCR testing with E. coli strains representing all 166 O-antigen forms, some randomly selected gram-negative bacteria, and Salmonella enterica serovar Adelaide, we showed that four O-antigen genes are highly specific to O111. This work provides the basis for a sensitive test for the rapid detection of E. coli O111. This is important both for decisions related to patient care, because early treatment may reduce the risk of life-threatening complications, and for the detection of sources of contaminat
O-antigen, Escherichia, Escherichia coli, gene cluster, sequencing
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 2
Aglycon: core
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_983931,SB_192,SB_7
Methods: PCR, DNA sequencing
Biosynthesis and genetic data: genetic data, biosynthesis data
NCBI Taxonomy refs (TaxIDs): 1055535Reference(s) to other database(s): GTC:G92229PR, GlycomeDB:
27270
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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