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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11: XN6P4

]

NCBI PubMed ID: 12784630
Publication DOI: 10.1271/bbb.67.869
Journal NLM ID: 9205717
Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Correspondence: inagaki

bio.mie-u.ac.jp
Institutions: Department of Life Science, Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu, Mie 514-8507, Japan

A histidine-tagged G protein of bacteriophage phi X174 (HisG) bound strongly with lipopolysaccharide (LPS) of Escherichia coli C, one of a phi X174-sensitive Ra strain. The dissociation constant, Kd, was measured to be 0.16 +/- 0.04 microM by fluorometric titration. HisG showed slightly less affinity to LPSs of the insensitive Rc and Rd 2 strains having shorter R-core polysaccharide sequences than that of the sensitive Ra strains. The difference between the two types of LPS was demonstrated by CD spectra; LPSs of the sensitive strains increased the signal intensity for beta-sheet, while the insensitive strains decreased it. The chemically degraded LPS derivatives lacking a hydrophobic lipid region showed much less affinity to HisG, indicating the importance of the lipid region of LPS for strong binding with HisG. On the other hand, since the degraded derivatives increased the intensity of CD spectra, the polysaccharide region is thought to contribute to the conformation change of the protein

Lipopolysaccharide, Escherichia coli, lipid, recognition, spike G protein, ϕX174

Structure type: oligomer
Location inside paper: p.872, fig.1 chemotype Ra
Aglycon: lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130658,IEDB_130659,IEDB_130670,IEDB_131186,IEDB_133751,IEDB_135818,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_150901,IEDB_151528,IEDB_153543,IEDB_190606,IEDB_2189047,IEDB_983931,SB_192,SB_7

Methods: de-O-acylation, de-N-O-acylation, CD, fluorometric titration

Related record ID(s): 31637, 31638, 31639
NCBI Taxonomy refs (TaxIDs): 498388
Reference(s) to other database(s): GTC:G04076OT
Show glycosyltransferases

There are 6 chemically distinct structures. Please, select:

NCCOP(=O)(O)OP(=O)(O)O[C@H]1[C@H](O)[C@H](O)[C@@H](O[C@H]2[C@@H]([C@@H](CO)OP(=O)(O)OP(=O)(O)OCCN)O[C@@](OC(=O)[C@@]3(O)C[C@@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O3)(C(=O)O)C[C@H]2O[C@]2(C(=O)O)C[C@@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O2)O[C@@H]1[C@@H](O)CO[C@H]1O[C@H]([C@@H](O)CO[C@H]2O[C@H]([C@@H](O)CO)[C@@H](O)[C@H](O)[C@@H]2O)[C@@H](OP(=O)(O)O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H]3O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)[C@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize