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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)

The structure was elucidated in this paper
NCBI PubMed ID: 10981707
Journal NLM ID: 7705721
Publisher: Blackwell Publishing
Correspondence: evguenii.vinogradov

nrc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ont. Canada, K1A 0R6

A strain of Citrobacter sedlakii showing serological cross-reaction with Escherichia coli O157 antisera was demonstrated to produce a lipopolysaccharide O-antigen having an identical structure with that of the E. coli O157 O-antigen. A strain of Citrobacter freunndii showing similar cross-reaction with E. coli O157 specific monoclonal antibody was shown to produce a lipopolysaccharide O-antigen composed of a trisaccharide repeating unit having the structure [ 2)-α-D Rhap-(1-3)-β-D-Rhap-(1-4)-β-D-Glcp-(1-]. This O-antigen differs from that of the E. coli O157 O-antigen and also lacks a component 2-substituted 4-amino-4,6-dideoxy-α-D-mannopyranosyl residue implicated as the common epitope in the lipopolysaccharide O-antigens of previously investigated bacterial species showing serological cross-reactivity with E. coli O157 antisera. The C freundii O-antigen presents an interesting example of structural mimicry within a bacterial polysaccharide antigen.

Lipopolysaccharide, Escherichia coli O157:H7, antigens, O-polysaccharide, Citrobacter, Citrobacter freundii, cross-reaction

Structure type: suggested polymer biological repeating unit
Location inside paper: Fig. 1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_136045,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_146664,IEDB_152214,IEDB_174333,IEDB_885822,IEDB_983931,SB_192,SB_86

Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, ELISA, GLC, immunodiffusion assays
Biological activity: cross-reactive with E. coli O157
Biosynthesis and genetic data: serological data, immunological data
Comments, role: chemical repeat frame is different in the paper

Related record ID(s): 1619, 7417, 8611, 8630, 8702, 8988, 20690, 27347, 30269, 30358, 105778, 108677
NCBI Taxonomy refs (TaxIDs): 67826
Reference(s) to other database(s): GTC:G60704LX, GlycomeDB:27267
Show glycosyltransferases

There is only one chemically distinct structure:

*O[C@@H]1[C@@H](O[C@H]2[C@H](O)[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](O[C@H]4[C@@H](O)[C@@H](CO)O[C@H](*)[C@@H]4NC(C)=O)O[C@@H]3CO)O[C@@H](C)[C@H]2O)O[C@H](C)[C@@H](NC(C)=O)[C@@H]1O

SVG MW SMILES 3D mol Ionize