Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Campylobacter jejuni [ICD11:
XN4Q5 
]
NCBI PubMed ID: 10075420Journal NLM ID: 0376646Publisher: Washington, DC: Kluwer Academic/Plenum Publishers
Correspondence: ket!le.ac.uk
Institutions: Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK
A region encoding ORFs with homology to known lipopolysaccharide (LPS) biosynthesis genes was isolated from two strains of Campylobacter jejuni. One of the strains produces LPS, but the second strain is reported to produce only lipooligosaccharide (LOS) and therefore lacks the O-chain. The two strains shared six predicted ORFs, but an additional ORF, orfE, of unknown function was identified in the LOS-producing strain. Mutation of the shared wbeE (rfbE) homologue (orfF) or deletion of five of the seven genes reduced core reactivity with specific antiserum without affecting O-chain production. Mutation of either the capD homologue (orfG) or the unique orfE had no detectable effect on LOS or LPS production. The presence or absence of orfE in 36 isolates of C. jejuni did not correlate with LOS/LPS phenotype or serotype. However, after insertion of orfE into a LPS-producing orfE-negative strain the O-chain ladder was no longer detectable on Western blots. We were not able to disrupt the wbaP (rfbP) homologue (orfC) in C jejuni
Lipopolysaccharide, genetics, Campylobacter, mutation, distribution
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1
Compound class: CPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_151528,IEDB_151531,IEDB_190606,SB_173,SB_7
Methods: PCR, DNA sequencing, SDS-PAGE
Biological activity: serological data
Biosynthesis and genetic data: biosynthesis data, genetic data
Comments, role: polysaccharide, is linked to the terminal GalNAc of the LPS core
Related record ID(s): 8662, 8714, 10261, 10307, 108584
NCBI Taxonomy refs (TaxIDs): 197Reference(s) to other database(s): GTC:G71039BI, GlycomeDB:
3492
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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