Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 9570406Journal NLM ID: 8712028Publisher: Blackwell Publishing
Correspondence: j45811a
nucc.cc.nagoya-u.ac.jp
Institutions: Biosystems, School of Informatics and Sciences, Nagoya University, Nagoya, Japan
WbdA (previously MtfA) is one of the mannosyltransferases encoded within the Escherichia coli O9a wb* gene cluster. It is composed of two domains of similar size, connected by an alpha-helix chain. Elimination of the C-terminal half by transposon insertion or gene deletion caused synthesis of an altered structural O-polysaccharide consisting only of α-1,2-linked mannose. O9a polysaccharide synthesis was restored by the C-terminal half of WbdA in trans. No membrane incorporation of mannose from GDP mannose was observed in a strain carrying only the gene for truncated WbdA. For mannose incorporation, it was necessary to introduce both wbdB and wbdC genes into the strain. Therefore, it is likely that the N-terminal half of truncated WbdA synthesizes the altered O-polysaccharide together with other mannosyltransferases which participate in the initial reactions of the O9a polysaccharide synthesis. Both N- and C-terminal domains of WbdA are required for the synthesis of the complete E. coli O9a polysaccharide. The chi sequence location between the two domains and homology plot analyses of the wbdA and the WbdA protein suggested that the wbdA gene might have arisen by fusion of two independent genes.
synthesis, gene, polysaccharide, Escherichia, Escherichia coli, cluster, gene cluster, domain, domains
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141111,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_174840,IEDB_241100,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72
Methods: NMR
Biosynthesis and genetic data: genetic data, biochemical data
Comments, role: published polymerization frame was shifted for conformity with other records.
Related record ID(s): 923, 7236, 10713, 20623, 23433
NCBI Taxonomy refs (TaxIDs): 1010797Reference(s) to other database(s): GTC:G54439QN, GlycomeDB:
25319
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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