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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_140630,IEDB_141582,IEDB_141584,IEDB_153207,IEDB_153510,IEDB_153749,IEDB_423153,IEDB_885822

• Article ID: 249
  Gwozdzinski K, Pieniazek A, Kaca W "Lipopolysaccharide from Proteus mirabilis O29 induces changes in red blood cell membrane lipids and proteins" - International Journal of Biochemistry and Cell Biology 35(3) (2003) 333-338
  

  Alterations in red blood cell (RBC) plasma membranes, i.e. in lipids and proteins, and osmotic fragility of these cells after treatment with Proteus mirabilis O29 endotoxin (lipolysaccharide (LPS)) were examined using a spin labelling method. At the highest concentration of LPS, insignificantly decreased fluidity of membrane lipids was observed. Changes in conformation of membrane proteins were determined by two covalently bound spin labels, 4-maleimido-2,2,6,6-tetramethylpiperidine- 1-oxyl (MSL) and 4-iodoacetamido-2,2,6,6-tetramethylpiperidine-1-oxyl (ISL). The analysis of spectra of MSL and ISL showed modifications in membrane proteins in red blood cells treated with the highest concentration of lipopolysaccharide. On the other hand, in the case of isolated membranes, disturbances in membrane were observed for all concentrations of LPS. The alterations in membrane lipids and proteins are paralleled in a significant rise in osmotic fragility of RBCs upon endotoxin treatment. These results provide experimental evidence that P. mirabilis O29 LPS causes deleterious changes in membranes of human red blood cells. They show that action of lipopolysaccharide mainly concerns the membrane cytoskeleton

  Lipopolysaccharide, conformation, LPS, blood, human, analysis, cell, molecular, lipid, protein, endotoxin, Proteus, Proteus mirabilis, biophysics, modification, method, treatment, action, alteration, blood cells, bound, case, Cell Membrane, cells, change, concentration, experimental, fluidity, lipids, membrane, Membrane Lipids, Membrane Proteins, membranes, osmotic, plasma, proteins, red blood cells

  NCBI PubMed ID: 12531246
  Journal NLM ID: 9508482
  Publisher: Amsterdam: Elsevier
  Correspondence: kgwozdz@biol.uni.lodz.pl
  Institutions: Department of Molecular Biophysics, University of Lodz, Polish Academy of Sciences, 90-237, Lodz, Poland, Institute of Microbiology, University of Lodz, Polish Academy of Sciences, 90-237 Lodz, Poland, Center of Microbiology and Virology, Polish Academy of Sciences, 90-237 Lodz, Poland
  
   
  
  Proteus mirabilis O29
  CSDB ID 6886 (all data & tools)
• Article ID: 494
  Torzewska A, Kocharova NA, Maszewska A, Knirel YA, Rozalski A "Serological characterization of the O-specific polysaccharide of Providencia alcalifaciens O23" - Archivum Immunologiae et Therapiae Experimentalis 52(1) (2004) 43-49
  

  INTRODUCTION: The genus Providencia belongs to the Enterobacteriaceae family and currently consists of five species: P. alcalifaciens, P. heimbachae, P. rettgerii, P. rustigianii and P. stuartii. The serological classification scheme of P. alcalifaciens, P. rustigianii and P. stuartii includes 63 O-serogroups and 30 H-serogroups. The O-antigenic specificity is defined by the structure of the O-antigen (O-specific polysaccharide--OPS), a part of the lipopolysaccharide (LPS, endotoxin), one of the major components of the outer membrane of gram-negative bacteria and an important virulence factor of these bacteria. Among the bacteria of the Enterobacteriaceae family, the genus Providencia is one of the least studied in respect to its LPS structure and antigenic specificity. Studies of the chemical structures and the serological specificity of the O-antigens aim at the elucidation of the molecular basis of the serological classification of Providencia sp. MATERIALS AND METHODS: LPS and alkali-treated LPS of P. alcalifaciens O23 and serologically related P. rustigianii O14, P. mirabilis O13 and P. myxofaciens as well as O-antiserum against P. alcalifaciens O23 were used. Serological characterization of P. alcalifaciens O23 O-specific polysaccharide was done by use enzyme immunosorbent assay (EIA), passive hemolysis test (PHT) as well as by inhibition and sodium deoxycholate polyacrylamide gel electrophoresis (DOC-PAGE) of LPS and Western blot. RESULTS AND CONCLUSIONS: The OPS of P. alcalifaciens, O23, contains an N-(D-glucuronoyl)-N-[(R)-1-carboxyethyl]-L-lysine residue (GlcAAlaLys). The LPS of P. alcalifaciens, O23, and other LPSs containing AlaLys from Providencia and Proteus strains were tested with rabbit anti-P. alcalifiaciens O23 serum. The serological data showed that a GlcAAlaLys-associated epitope plays a role as an antigenic determinant in the P. alcalifaciens O23 OPS and revealed the particular importance of glucuronic acid and the carboxyethyl group for the binding of O23-specific antibodies.

  Lipopolysaccharide, structure, characterization, polysaccharide, O-antigen, O-specific, O-specific polysaccharide, Providencia, Providencia alcalifaciens, serological, O-serogroups, Ne-[(R)-1-carboxyethyl]-L-lysine

  NCBI PubMed ID: 15053232
  Journal NLM ID: 0114365
  Publisher: Basel, Boston: Birkhaüser
  Correspondence: rozala@biol.uni.lodz.pl
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Microbiology and Immunology, University of Lodz, Lodz, Poland
  
   
  
  Proteus mirabilis O29
  CSDB ID 9318 (all data & tools)
• Article ID: 876
  Kocharova NA, Maszewska A, Zatonsky GV, Bystrova OV, Ziolkowski A, Torzewska A, Shashkov AS, Knirel YA, Rozalski A "Structure of the O-polysaccharide of Providencia alcalifaciens O21 containing 3-formamido-3,6-dideoxy-D-galactose" - Carbohydrate Research 338(13) (2003) 1425-1430
  

  The O-polysaccharide (O-antigen) of Providencia alcalifaciens O21 was obtained by mild acid degradation of the lipopolysaccharide and studied by chemical methods and NMR spectroscopy. It was found that the polysaccharide is built up of branched pentasaccharide repeating units with a terminal residue of 3-formamido-3,6-dideoxy-D-galactose (D-Fuc3NFo) and has the following structure: [structure: see text]. Anti-P. alcalifaciens O21 serum cross-reacted with the O-antigen of Proteus vulgaris O47, which contains a GalNAc trisaccharide similar to that present in the P. alcalifaciens O21 O-polysaccharide.

  structure, polysaccharide, acid, O-polysaccharide, O polysaccharide, O-specific, O-specific polysaccharide, Providencia, Providencia alcalifaciens

  NCBI PubMed ID: 12801716
  Publication DOI: 10.1016/S0008-6215(03)00178-2
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: rozala@biol.uni.lodz.pl
  Institutions: Institute of Microbiology and Immunology, University of Lodz, Lodz, Poland
  Methods: methylation, NMR, acid hydrolysis, N-deformylation/N-trideuterioacetylation
  
   
  
  Proteus mirabilis O29
  CSDB ID 2957 (all data & tools)
• Article ID: 1094
  Perepelov AV, Shashkov AS, Babicka D, Senchenkova SN, Bartodziejska B, Rozalski A, Knirel YA "Structure of the O-specific polysaccharide of the bacterium Proteus vulgaris O23" - Biochemistry (Moscow) 65(9) (2000) 1055-1059
  

  An O-specific polysaccharide was obtained by mild acid degradation of P. mirabilis O29 lipopolysaccharide (LPS) and found to contain 2-acetamido-2-deoxy-D-galactose and D-glucuronic acid (D-GlcA) in the ratio 3:1. Studies of the polysaccharide by 1H- and 13C NMR spectroscopy including two-dimensional correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), nuclear Overhauser effect spectroscopy (NOESY), and H-detected 1H,13C-heteronuclear multiple-quantum coherence (HMQC) experiments demonstrated the following structure of the branched tetrasaccharide repeating unit:

  O-antigen; lipopolysaccharide; bacterial polysaccharide; structure; Proteus mirabilis

  NCBI PubMed ID: 10713543
  Journal NLM ID: 0376536
  Publisher: Nauka/Interperiodica
  Correspondence: knirel@ioc.ac.ru
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, 117913 Moscow, Leninsk Prospekt 47, Microbiology and Virology Ceter, PAN, Lodz, Poland, Institute of Mcrobiology and Immunology, University of Lodz, Poland
  Methods: NMR-2D, NMR, sugar analysis
  
   
  
  Proteus mirabilis O29
  CSDB ID 4035 (all data & tools)
• Article ID: 1095
  Perepelov AV, Shashkov AS, Senchenkova SN, Knirel YA, Literacka E, Kaca W "Structure of the O-specific polysaccharide of the bacterium Proteus mirabilis O29" - Biochemistry (Moscow) 65(2) (2000) 176-179
  

  An O-specific polysaccharide was obtained by mild acid degradation of P. mirabilis O29 lipopolysaccharide (LPS) and found to contain 2-acetamido-2-deoxy-D-galactose and D-glucuronic acid (D-GlcA) in the ratio 3:1. Studies fo the polysaccharide by 1H- and 13C NMR spectroscopy including two-dimensional correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), nuclear Overhauser effect spectroscopy (NOESY), and H-detected 1H,13C-heteronuclear multiple-quantum coherence (HMQC) experiments demonstrated the following structure of the branched tetrasaccharide repeating unit: (see formula in text).

  Lipopolysaccharide, LPS, structure, strain, structural, characterization, polysaccharide, O-antigen, bacteria, O-specific, O-specific polysaccharide, Proteus, Proteus mirabilis, serological, chemical, immunochemical

  NCBI PubMed ID: 10713543
  Journal NLM ID: 0376536
  Publisher: Nauka/Interperiodica
  Correspondence: perepel@ioc.ac.ru
  Institutions: Institute of Microbiology and Immunology, University of Lodz, Poland, Center of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland, N.D. Zelinsky Institute of Organic Chamisry, Russian Academy of Sciences, Leninsky pr. 47, Moscow, 119991 Russia
  Methods: NMR-2D, NMR, sugar analysis, determination of absolute configuration
  
   
  
  Proteus mirabilis O29
  CSDB ID 4036 (all data & tools)
• Article ID: 1467
  Knirel YA, Kaca W, Rozalski A, Sidorczyk Z "Structure of the O-antigenic polysaccharides of Proteus bacteria" - Polish Journal of Chemistry 73 (1999) 895-907
  

  Data on the composition and structure of the O-specific polysaccharides (O-antigens) of the lipopolysaccharides of the genus Proteus are summarized and discussed as the molecular basis for serotyping of these medically important bacteria.

  structure, O-antigen, Proteus, Bacterial polysaccharide, epitope specificity

  Journal NLM ID: 7901356
  WWW link: http://www.ichf.edu.pl/pjch/pj-1999/pj0699.htm#0895
  Publisher: Państwowe Wydawnictwo Naukowe
  Correspondence: knirel@ioc.ac.ru
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences,Leninsky Prospekt 47, Moscow, Russia, Institute of Microbiology and Immunology, University of ŁódźŸ, Banacha 12/16, 90-237 ŁódŸź, Poland, Center of Microbiology and Virology, Polish Academy of Sciences, Lodowa 106, 93-232 ŁódźŸ, Poland
  
   
  
  Proteus mirabilis O29
  CSDB ID 31867 (all data & tools)
• Article ID: 1553
  Perepelov AV, Zablotni A, Shashkov AS, Knirel YA, Sidorczyk Z "Structure of the O-polysaccharide and serological studies of the lipopolysaccharide of Proteus mirabilis 2002" - Carbohydrate Research 340 (2005) 2305-2310
  

  The structure of the O-polysaccharide of the lipopolysaccharide of Proteus mirabilis 2002 was elucidated by chemical methods and (1)H and (13)C NMR spectroscopy. It was found that the polysaccharide consists of branched pentasaccharide repeating units having the following structure: The O-polysaccharide of P. mirabilis 2002 has a common tetrasaccharide fragment with that of P. mirabilis 52/57 from serogroup O29, and the lipopolysaccharides of the two strains are serologically related. Therefore, based on the structural and serological data, we propose to classify P. mirabilis 2002 into the Proteus O29 serogroup as a subgroup O29a,29b.

  Lipopolysaccharide, structure, polysaccharide, O-polysaccharide, O polysaccharide, O-specific, O-specific polysaccharide, Proteus, Proteus mirabilis, serological, serogroup, classification, preparation, genomospecies

  NCBI PubMed ID: 16084933
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Department of General Microbiology, Institute of Microbiology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland
  Methods: methylation, NMR, sugar analysis
  
   
  
  Proteus mirabilis O29
  CSDB ID 10358 (all data & tools)
• Article ID: 4802
  Siwinska M, Levina EA, Ovchinnikova OG, Drzewiecka D, Shashkov AS, Rozalski A, Knirel YA "Classification of a Proteus penneri clinical isolate with a unique O-antigen structure to a new Proteus serogroup, O80" - Carbohydrate Research 407 (2015) 131-136
  

  Proteus penneri is an opportunistic pathogen, which may cause severe diseases, most frequently urinary tract infections in immunocompromised patients. P. penneri Br 114 exhibiting a good swarming growth ability as an S-form strain was isolated from a wound of a patient in Lodz, Poland. Serological studies using ELISA and Western blotting and chemical analyses along with (1)H and (13)C NMR spectroscopy showed that the O-antigen (O-polysaccharide) of this strain is unique among the known Proteus serotypes O1-O79. It possesses a linear pentasaccharide repeating unit containing a partially O-acetylated amide of D-glucuronic acid (GlcA) with L-serine having the following structure: [structure: see text]. These data are a basis for creating a new Proteus serogroup, O80, so far represented by the single Br 114 isolate. The O80 is the 21st O-serogroup containing P. penneri strains and the fourth serogroup based on Proteus spp. clinical isolates from Lodz, Poland.

  Lipopolysaccharide, O-antigen, Proteus penneri, serological classification, bacterial polysaccharide structure

  NCBI PubMed ID: 25771295
  Publication DOI: 10.1016/j.carres.2015.02.003
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: domkam@biol.uni.lodz.pl
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, 90-237 Łódź, Poland, Higher Chemical College of the Russian Academy of Sciences, 125047 Moscow, Russia, Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, 90-237 Łódź, Poland
  Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, SDS-PAGE, ELISA, GLC, mild acid hydrolysis, Western blotting, de-O-acetylation, composition analysis, GPC
  
   
  
  Proteus mirabilis O29a
  CSDB ID 30882 (all data & tools)