Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
];
infection due to Salmonella enterica [ICD11:
XN5VC ];
infection due to Citrobacter freundii [ICD11:
XN0M3 ]
NCBI PubMed ID: 15375135Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: p.reeves
angis.usyd.edu.au
Institutions: School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
Escherichia coli O157, Salmonella enterica O30, and Citrobacter freundii F90 have identical O-antigen structures, as do E. coli O55 and S. enterica O50. The O-antigen gene cluster sequences for E. coli O157 and E. coli O55 have been published, and the genes necessary for O-antigen biosynthesis have been identified, although transferase genes for glycosidic linkages are only generic and have not been allocated to specific linkages. We determined sequences for S. enterica O30 and C. freundii F90 O-antigen gene clusters and compared them to the sequence of the previously described E. coli O157 cluster. We also determined the sequence of the S. enterica O50 O-antigen gene cluster and compared it to the sequence of the previously described E. coli O55 cluster. For both the S. enterica O30-C. freundii F90-E. coli O157 group and the S. enterica O50-E. coli O55 group of O antigens, the gene clusters have identical or nearly identical organizations. The two sets of gene clusters had comparable overall levels of similarity in their genes, which were lower than the levels determined for housekeeping genes for these species, which were 55 to 65% for the genes encoding glycosyltransferases and O-antigen processing proteins and 75 to 93% for the nucleotide-sugar pathway genes. Nonetheless, the similarity of the levels of divergence in the five gene clusters required us to consider the possibility that the parent gene cluster for each structure was in the common ancestor of the species and that divergence is faster than expected for the common ancestor hypothesis. We propose that the identical O-antigen gene clusters originated from a common ancestor, and we discuss some possible explanations for the increased rate of divergence that is seen in these genes.
structure, genetics, Escherichia coli, Escherichia coli O157, Molecular Sequence Data, gene cluster, O-antigens, glycosyltransferases, Salmonella enterica, Citrobacter freundii
Structure type: suggested polymer biological repeating unit
Location inside paper: Fig. 1A
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_136045,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_146664,IEDB_152214,IEDB_174333,IEDB_885822,IEDB_983931,SB_192,SB_86
Biosynthesis and genetic data: genetic data
Related record ID(s): 1619, 7417, 8630, 8701, 8702, 9058, 20690, 21404, 27347, 30269, 30358, 105778, 108677
NCBI Taxonomy refs (TaxIDs): 1045010,
28901,
546Reference(s) to other database(s): GTC:G60704LX, GlycomeDB:
27267
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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