Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 14981698Journal NLM ID: 8204476Publisher: Wiley-VCH
Correspondence: volpi
unimo.it
Institutions: Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
A rapid, highly sensitive and reproducible high-performance capillary electrophoresis (HPCE) method (electrokinetic chromatography with sodium dodecyl sulfate) is described for the determination of the polysaccharide from the uropathogenic Escherichia coli K4 bacteria (05:K4:H4) and its defructosylated product. The two polyanions, K4 and defructosylated K4, are separated and readily determined within 30 min on an uncoated fused-silica capillary using normal polarity at 20 kV and detection at 200 nm. A linear relationship was found for the two polysaccharides over a wide range of concentrations, from approximately 30 ng (0.5 microg/microL) to 210 ng (3.5 microg/microL). The described method was used to evaluate the defructosylation process of K4 under drastic acid conditions.
chondroitin, glycosaminoglycans, K4 polysaccharide, fructose, capillary electrophoresis
Structure type: polymer chemical repeating unit
Location inside paper: p.692
Trivial name: chondroitin
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_137473,IEDB_140630,IEDB_153510,IEDB_423153
Comments, role: defructosylated capsular polysaccaride, separation on analogous sulfated polymers
Related record ID(s): 5722, 6606, 6937, 8800, 8901, 9301, 10190
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G43702JT, GlycomeDB:
2738
Show glycosyltransferases
There is only one chemically distinct structure:
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