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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Campylobacter jejuni [ICD11: XN4Q5

]

NCBI PubMed ID: 14742567
Journal NLM ID: 0246127
Publisher: American Society for Microbiology
Correspondence: michel.gilbert

nrc.cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council Canada, Ottawa, Ontario, Canada

Campylobacter jejuni GB11, a strain isolated from a patient with Guillain-Barre syndrome, has been shown to be genetically closely related to the completely sequenced strain C. jejuni NCTC 11168 by various molecular typing and serotyping methods. However, we observed that the lipooligosaccharide (LOS) biosynthesis genes strongly diverged between GB11 and NCTC 11168. We sequenced the LOS biosynthesis locus of GB11 and found that it was nearly identical to the class A LOS locus from the C. jejuni HS:19 Penner serotype strain (ATCC 43446). Analysis of the DNA sequencing data showed that a horizontal exchange event involving at least 14.26 kb had occurred in the LOS biosynthesis locus of GB11 between galE (Cj1131c in NCTC 11168) and gmhA (Cj1149 in NCTC 11168). Mass spectrometry of the GB11 LOS showed that GB11 expressed an LOS outer core that mimicked the carbohydrate portion of the gangliosides GM1a and GD1a, similar to C. jejuni ATCC 43446. The serum from the GB11-infected patient was shown to react with the LOS from both GB11 and ATCC 43446 but not with that from NCTC 11168. These data indicate that the antiganglioside response in the GB11-infected patient was raised against the structures synthesized by the acquired class A LOS locus.

biosynthesis, lipopolysaccharides, structure, core, Lipooligosaccharide, chemistry, gene, genetics, human, LOS, metabolism, microbiology, DNA, strain, Support, Non-U.S.Gov't, serotype, analysis, carbohydrate, molecular, Research, locus, Campylobacter, Campylobacter jejuni, class, ganglioside, gangliosides, Guillain-Barre syndrome, immunology, Molecular Sequence Data, response, syndrome, spectrometry, biological, DNA sequencing, mass spectrometry, sequencing, method, methods, serotyping, Base Sequence, serum, acquisition, Chromosome Mapping, event, exchange, galE, U.S.Gov't, Non-P.H.S., typing, PDF

Structure type: oligomer
Location inside paper: Fig. 2B
Compound class: LOS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130648,IEDB_130650,IEDB_131186,IEDB_134627,IEDB_135818,IEDB_136044,IEDB_136794,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_151528,IEDB_167072,IEDB_190606,IEDB_2189047,IEDB_742245,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_23,SB_24,SB_25,SB_39,SB_68,SB_7,SB_8,SB_84,SB_88,SB_96

Biosynthesis and genetic data: genetic data

Related record ID(s): 8970, 9045, 9047, 9048
NCBI Taxonomy refs (TaxIDs): 197
Reference(s) to other database(s): GTC:G59369VC
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There are 2 chemically distinct structures. Please, select:

CC(=O)N[C@@H]1[C@@H](O)C[C@](O[C@H]2[C@@H](O[C@@H]3O[C@H](CO)[C@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@H]3NC(C)=O)[C@@H](CO)O[C@@H](O[C@H]3[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]4[C@H](O[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@@H](O[C@@H]5[C@H](O)[C@@H](O[C@@H]6[C@H](O)CC(O)(C(=O)O)O[C@@H]6[C@H](O)CO)O[C@H]([C@H](CO)OP(=O)(O)OCCN)[C@H]5O[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)O[C@H]([C@@H](O)CO)[C@H]4O)[C@@H]3O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)[C@@H]2O)(C(=O)O)O[C@H]1[C@H](O)[C@H](O)CO

SVG MW SMILES 3D mol Ionize