Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 9373936Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Microbiology, University of Sydney, New South Wales 2006, Australia, School of Chemistry, Macquairie University, New South Wales 2109, Australia
The chemical structure of the 016 antigen from the lipopolysaccharide of Escherischia coli strain P4 has been determined. Comparison with the structures of other O16 antigens and that of the O17 antigen explains the previously reported cross-reaction og O-antigen from the O16 strain K-12 with anti-017 antibody [D. Liu and P.R, Reeves Microlnolosy, 140(1994)49-57]
Escherichia, Escherichia coli, NMR spectroscopy, polysaccharide structure, relationship
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1
Contained glycoepitopes: IEDB_130422,IEDB_136095,IEDB_136105,IEDB_137340,IEDB_137472,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_158539,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983931,SB_192
Methods: methylation, periodate oxidation, NMR-2D, acid hydrolysis, composition analysis
Biological activity: serological data
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G75022IJ
Show glycosyltransferases
NMR conditions: in D2O at 303(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,6 bDGalf 107.20 87.56 76.73 83.43 71.50 64.15
3,3 aDGlcp 98.17 72.50 74.14 70.16 71.50 66.98
3,2 Ac ? 23.18
3 aLRhap 102.04 69.18 78.11 71.58 70.56 17.80
2 Ac ? 23.18
4 50%Ac
6 50%Ac
aDGlcpN 97.79 54.16 80.46 69.43 74.02 61.78
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,6 | bDGalf | 107.20 | 87.56 | 76.73 | 83.43 | 71.50 | 64.15 |
| 3,3 | aDGlcp | 98.17 | 72.50 | 74.14 | 70.16 | 71.50 | 66.98 |
| 3,2 | Ac | ? | 23.18 | |
| 3 | aLRhap | 102.04 | 69.18 | 78.11 | 71.58 | 70.56 | 17.80 |
| 2 | Ac | ? | 23.18 | |
| 4 | 50%Ac | |
| 6 | 50%Ac | |
| | aDGlcpN | 97.79 | 54.16 | 80.46 | 69.43 | 74.02 | 61.78 |
|
![](nmr_drawer.py?width=750&height=130&nucleus=C&rotate_labels=false&spectrum=[{"s":107.2,"l":"1","c":[0,0,225]},{"s":87.56,"l":"2","c":[0,0,225]},{"s":76.73,"l":"3","c":[0,0,225]},{"s":83.43,"l":"4","c":[0,0,225]},{"s":71.5,"l":"5","c":[0,0,225]},{"s":64.15,"l":"6","c":[0,0,225]},{"s":98.17,"l":"1","c":[230,0,30]},{"s":72.5,"l":"2","c":[230,0,30]},{"s":74.14,"l":"3","c":[230,0,30]},{"s":70.16,"l":"4","c":[230,0,30]},{"s":71.5,"l":"5","c":[230,0,30]},{"s":66.98,"l":"6","c":[230,0,30]},{"s":23.18,"l":"2","c":[0,0,0]},{"s":102.04,"l":"1","c":[0,165,0]},{"s":69.18,"l":"2","c":[0,165,0]},{"s":78.11,"l":"3","c":[0,165,0]},{"s":71.58,"l":"4","c":[0,165,0]},{"s":70.56,"l":"5","c":[0,165,0]},{"s":17.8,"l":"6","c":[0,165,0]},{"s":23.18,"l":"2","c":[0,0,0]},{"s":97.79,"l":"1","c":[220,0,180]},{"s":54.16,"l":"2","c":[220,0,180]},{"s":80.46,"l":"3","c":[220,0,180]},{"s":69.43,"l":"4","c":[220,0,180]},{"s":74.02,"l":"5","c":[220,0,180]},{"s":61.78,"l":"6","c":[220,0,180]}])
The spectrum also has 2 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
Found 
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