Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 15178392Publication DOI: 10.1016/j.carres.2004.04.002Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: G. Widmalm <gw
organ.su.se>
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden
The structure of the O-antigen polysaccharide (PS) from the enteroaggregative Escherichia coli strain 396/C-1 has been determined. Sugar and methylation analyses together with (1)H and (13)C NMR spectroscopy were the main methods used. Inter-residue correlations were determined by (1)H,(1)H-NOESY, (1)H,(13)C-heteronuclear multiple-bond correlation and dipole-dipole cross-correlated relaxation experiments. The PS is composed of pentasaccharide repeating units with the following structure: Analysis of NMR data reveals that on average the PS consists of approximately 13 repeating units and indicates that the biological repeating unit contains an N-acetylglucosamine residue at its reducing end. This structure is different to that reported for the O-antigen polysaccharide from E. coli O126. Monospecific anti-E. coli O126 rabbit serum from The International Escherichia and Klebsiella Centre did not distinguish between the E. coli strain 396/C-1 and the E. coli O126 reference strain, neither in slide agglutination nor in an indirect enzyme immunoassay. Subsequent successful serotyping of the E. coli strain 396/C-1 showed it to be E. coli O126:K+:H27.
Lipopolysaccharide, NMR, Escherichia coli, serology, Enteroaggregative
Structure type: suggested polymer biological repeating unit
Location inside paper: p. 1494
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136045,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137485,IEDB_141794,IEDB_141807,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_152214,IEDB_153553,IEDB_174333,IEDB_190606,IEDB_983930,SB_154,SB_44,SB_7,SB_72,SB_86
Methods: methylation, NMR-2D, NMR, sugar analysis
Comments, role: previously reported structure, which seems to be incorrect
Related record ID(s): 9273, 20679, 21878, 117665
NCBI Taxonomy refs (TaxIDs): 2234098Reference(s) to other database(s): GTC:G86653XO, GlycomeDB:
27557
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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