Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 15297533Journal NLM ID: 7505564Publisher: American Society for Microbiology
Correspondence: wanglei
nankai.edu.cn
Institutions: TEDA School of Biological Sciences and Biotechnology, TEDA College, Nankai University, TEDA, Tianjin 300457, People's Republic of China
Screening for the Escherichia coli O serotype is the traditional test for identification of E. coli clones. The O-antigen gene cluster of the E. coli O114 type strain was sequenced, and 12 open reading frames were assigned functions on the basis of homology. By screening against all 186 E. coli and Shigella O serotypes, five genes specific to E. coli O114 were identified. A PCR assay based on the O-antigen-specific genes was developed and tested on 41 clinical isolates of E. coli O114. The PCR assay was shown to be highly specific and sensitive. When tested with pork and water samples, as few as 0.12 CFU of E. coli O114 g(-1) were detected. Thus, the PCR assays established in this study can be used to reliably identify E. coli O114 strains and may also be used to detect E. coli O114 strains in food, water, and other environmental samples.
PCR, Escherichia coli, gene cluster, Shigella, Serotypes, O-serotype, O-antigen-specific
Structure type: suggested polymer biological repeating unit
Location inside paper: p. 3800
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136044,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_142078,IEDB_149136,IEDB_150899,IEDB_150900,IEDB_151531,IEDB_190606,SB_137,SB_165,SB_166,SB_187,SB_195,SB_29,SB_7,SB_88
Methods: PCR, DNA sequencing
Enzymes that release or process the structure: wzx, wzy, wbuM, wbuO, and wbuP
Biosynthesis and genetic data: genetic data
Comments, role: chemical repeat frame is different in the paper
Related record ID(s): 20674, 22457, 25584, 28333, 29401, 108673, 122750, 137911
NCBI Taxonomy refs (TaxIDs): 562
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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