Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
];
infection due to Klebsiella oxytoca [ICD11:
XN7WL ]
NCBI PubMed ID: 15103651Journal NLM ID: 9504818Publisher: Chichester, UK: Wiley
Correspondence: lanzetta
unina.it
Institutions: Dipartimento di Chimica Organica e Industriale, Universita di Parma, Parco Area delle Scienze 17/a, I-43100 Parma, Italy, Dipartimento di Chimica Organica e Biochimica, Università€ di Napoli Federico II, Complesso Universitario Monte Sant’Angelo, Via Cinthya 4, I-80126 Naples, Italy
Lipid A is the endotoxic principle of the lipopolysaccharide fraction from Gram-negative bacteria. It is involved in the elicitation of cytokine production that leads to massive inflammation and to septic shock as a lethal consequence. For this reason, the structural elucidation of lipopolysaccharides from toxic Gram-negative bacteria is an important and complicated task, mainly owing to its natural heterogeneity. Here, a new methodology to infer the distribution of the primary and secondary acyl residues is described, based on electrospray ionization mass spectrometry (ESI-MS) of intact lipid A under high cone voltage in order to achieve in-source fragmentation. Under these conditions, acyl fragmentation is induced and a different regioselective cleavage of secondary fatty acids is observed in positive and negative ESI-MS, allowing the rapid identification of the lipid A structure.
lipid A, Gram-negative bacteria, electrospray ionization mass spectrometry, fatty acid distribution, in-source collision-induced dissociation
Structure type: oligomer ; 1796
Location inside paper: p.379, fig.1, p.381, fig.4
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141807,IEDB_151531,IEDB_176772,IEDB_534864
Methods: ESI-MS, mild acid hydrolysis, CID-MS
Related record ID(s): 31325, 31326
NCBI Taxonomy refs (TaxIDs): 562,
572
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error