Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 31539669Publication DOI: 10.1016/j.carres.2019.107814Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: J.J. Kenyon <johanna.kenyon
qut.edu.au>
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia, Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia, Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia, Central Scientific Research Institute of Epidemiology, Moscow, Russia, School of Life and Environmental Sciences, University of Sydney, Sydney, Australia
The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy. The CPS was found to consist of branched pentasaccharide repeating units containing only neutral sugars, and its composition and topology are closely related to those of the A. baumannii K116 CPS. The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1→6) linkage in K128 replacing a β-(1→4) linkage in K116. The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1→4)-d-Galp, and β-d-GalpNAc-(1→3)-d-Galp linkages between K116 units. The KZ-1093 genome was sequenced and the CPS biosynthesis gene cluster at the chromosomal K locus was designated KL128. Consistent with the CPS structures, KL128 differs from KL116 in one glycosyltransferase gene and the gene for the Wzy polymerase. In KL128, the gtr200 glycosyltransferase gene replaces gtr76 in KL116, and Gtr200 was therefore assigned to the different β-d-GalpNAc-(1→6)-d-Galp linkage in K128. Similarly, the WzyK128 polymerase could be assigned to the β-d-GalpNAc-(1→4)-d-Galp linkage between the K128 units.
Acinetobacter baumannii, capsular polysaccharide, glycosyltransferase, Wzy polymerase, K locus
Structure type: polymer chemical repeating unit
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_134624,IEDB_134627,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_147450,IEDB_151528,IEDB_190606,IEDB_742248,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_23,SB_24,SB_25,SB_7,SB_8,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, sugar analysis, chemical analysis, ion-exchange chromatography, bioinformatic analysis
Biosynthesis and genetic data: Gtr75,Gtr200, Gtr25, Gtr5, Wzy(K128)[ItrA2]
Related record ID(s): 1179
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G41835WJ
Show glycosyltransferases
NMR conditions: in D2O at 333 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,6,6,6 bDGlcp 104.3 74.7 77.4 71.3 77.5 62.5
3,6,6,2 Ac 175.7-176.3 23.7-24.1
3,6,6 bDGalpN 103.9 53.2 75.1 70.1 73.8 70.4
3,6 aDGalp 100.1 69.5 71.2 78.5 71.3 72.2
3 bDGalp 106.2 72.3 74.1 70.1 73.8 67.8
2 Ac 175.7-176.3 23.7-24.1
bDGalpN 103.5 53.8 81.5 69.4 76.1 62.7
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,6,6,6 bDGlcp 4.50 3.32 3.51 3.41 3.47 3.75-3.92
3,6,6,2 Ac - 2.03-2.07
3,6,6 bDGalpN 4.49 3.92 3.86 3.99 3.72 3.93-4.07
3,6 aDGalp 4.94 3.72 3.90 4.09 4.00 3.82-4.07
3 bDGalp 4.49 3.55 3.64 3.98 3.83 3.70-3.84
2 Ac - 2.03-2.07
bDGalpN 4.69 3.99 3.87 4.15 3.63 3.78-3.78
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,6,6,6 bDGlcp 104.3/4.50 74.7/3.32 77.4/3.51 71.3/3.41 77.5/3.47 62.5/3.75-3.92
3,6,6,2 Ac 23.7-24.1/2.03-2.07
3,6,6 bDGalpN 103.9/4.49 53.2/3.92 75.1/3.86 70.1/3.99 73.8/3.72 70.4/3.93-4.07
3,6 aDGalp 100.1/4.94 69.5/3.72 71.2/3.90 78.5/4.09 71.3/4.00 72.2/3.82-4.07
3 bDGalp 106.2/4.49 72.3/3.55 74.1/3.64 70.1/3.98 73.8/3.83 67.8/3.70-3.84
2 Ac 23.7-24.1/2.03-2.07
bDGalpN 103.5/4.69 53.8/3.99 81.5/3.87 69.4/4.15 76.1/3.63 62.7/3.78-3.78
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,6,6,6 | bDGlcp | 4.50 | 3.32 | 3.51 | 3.41 | 3.47 | 3.75
3.92 |
| 3,6,6,2 | Ac |
| 2.03
2.07 | |
| 3,6,6 | bDGalpN | 4.49 | 3.92 | 3.86 | 3.99 | 3.72 | 3.93
4.07 |
| 3,6 | aDGalp | 4.94 | 3.72 | 3.90 | 4.09 | 4.00 | 3.82
4.07 |
| 3 | bDGalp | 4.49 | 3.55 | 3.64 | 3.98 | 3.83 | 3.70
3.84 |
| 2 | Ac |
| 2.03
2.07 | |
| | bDGalpN | 4.69 | 3.99 | 3.87 | 4.15 | 3.63 | 3.78
3.78 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,6,6,6 | bDGlcp | 104.3 | 74.7 | 77.4 | 71.3 | 77.5 | 62.5 |
| 3,6,6,2 | Ac | 175.7
176.3 | 23.7
24.1 | |
| 3,6,6 | bDGalpN | 103.9 | 53.2 | 75.1 | 70.1 | 73.8 | 70.4 |
| 3,6 | aDGalp | 100.1 | 69.5 | 71.2 | 78.5 | 71.3 | 72.2 |
| 3 | bDGalp | 106.2 | 72.3 | 74.1 | 70.1 | 73.8 | 67.8 |
| 2 | Ac | 175.7
176.3 | 23.7
24.1 | |
| | bDGalpN | 103.5 | 53.8 | 81.5 | 69.4 | 76.1 | 62.7 |
|
There is only one chemically distinct structure:
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