Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ];
infection due to Campylobacter jejuni [ICD11:
XN4Q5 ]
The structure was elucidated in this paperNCBI PubMed ID: 33621246Publication DOI: 10.1371/journal.pone.0247305Journal NLM ID: 101285081Publisher: San Francisco, CA: Public Library of Science
Correspondence: frederic.m.poly.civ
mail.mil
Institutions: Dept. of Chemistry, University of Guelph, Guelph, Ontario, Canada, Naval Medical Research Center, Silver Spring, Maryland, United States of America
The Campylobacter jejuni capsule type HS1 complex is one of the most common serotypes identified worldwide, and consists of strains typing as HS1, HS1/44, HS44 and HS1/8. The capsule structure of the HS1 type strain was shown previously to be composed of teichoic-acid like glycerol-galactosyl phosphate repeats [4-)-α-D-Galp-(1-2)-Gro-(1-P-] with non-stoichiometric fructose branches at the C2 and C3 of Gal and non-stoichiometric methyl phosphoramidate (MeOPN) modifications on the C3 of the fructose. Here, we demonstrate that the capsule of an HS1/44 strain is identical to that of the type strain of HS1, and the capsule of HS1/8 is also identical to HS1, except for an additional site of MeOPN modification at C6 of Gal. The DNA sequence of the capsule locus of an HS44 strain included an insertion of 10 genes, and the strain expressed two capsules, one identical to the HS1 type strain, but with no fructose branches, and another composed of heptoses and MeOPN. We also characterize a HS1 capsule biosynthesis gene, HS1.08, as a fructose transferase responsible for the attachment of the β-D-fructofuranoses residues at C2 and C3 of the Gal unit. In summary, the common component of all members of the HS1 complex is the teichoic-acid like backbone that is likely responsible for the observed sero-cross reactivity.
biosynthesis, structure, capsular polysaccharide, Campylobacter jejuni, capsule, teichoic acid
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1, Fig. 3, p. e0247305-9
Compound class: CPS
Contained glycoepitopes: IEDB_130695,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, DNA sequencing, GC-MS, SDS-PAGE, 31P NMR, GC, composition analysis, mutation analysis, SEC
Comments, role: NMR data of HS1/44 defructosylated CPS.
Related record ID(s): 9701, 9702, 10910
NCBI Taxonomy refs (TaxIDs): 197Reference(s) to other database(s): GTC:G71806EN
Show glycosyltransferases
NMR conditions: in D2O at 295 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
0,2 aDGalp 98.1 68.2 68.5 74.4 70.6 60.6
0 xDGro 64.3 77.1 61.1
P
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
0,2 aDGalp 5.21 3.89 3.99 4.53 4.18 3.74
0 xDGro 4.05-4.11 3.97 3.78
P
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
0,2 aDGalp 98.1/5.21 68.2/3.89 68.5/3.99 74.4/4.53 70.6/4.18 60.6/3.74
0 xDGro 64.3/4.05-4.11 77.1/3.97 61.1/3.78
P
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 0,2 | aDGalp | 5.21 | 3.89 | 3.99 | 4.53 | 4.18 | 3.74 |
| 0 | xDGro | 4.05
4.11 | 3.97 | 3.78 | |
| | P | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 0,2 | aDGalp | 98.1 | 68.2 | 68.5 | 74.4 | 70.6 | 60.6 |
| 0 | xDGro | 64.3 | 77.1 | 61.1 | |
| | P | |
|
There is only one chemically distinct structure:
Found 
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