Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Enterobacter cloacae [ICD11:
XN3YM 
]
The structure was elucidated in this paperNCBI PubMed ID: 34619615Publication DOI: 10.1016/j.carres.2021.108440Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: andreivperepelov
gmail.com
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, China
The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G3422 and studied by chemical methods, including sugar analyses, Smith degradation, and solvolysis with anhydrous trifluoroacetic acid, along with 1H and 13C NMR spectroscopy. The following structure of the branched tetrasaccharide repeating unit was established: The O-antigen gene cluster of Enterobacter cloacae G3422 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in a good agreement with the O-antigen structure.
Lipopolysaccharide, O-antigen, bacterial polysaccharide structure, O-antigen gene cluster, Enterobacter cloacae
Structure type: polymer chemical repeating unit
Location inside paper: table 1, p. 108440-3, DPS
Compound class: O-antigen
Contained glycoepitopes: IEDB_130701,IEDB_135813,IEDB_137340,IEDB_141807,IEDB_144983,IEDB_151531,IEDB_152206,IEDB_164174,IEDB_983930,SB_197,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, sugar analysis, DNA techniques, 31P NMR, GLC, mild acid hydrolysis, GPC, function analysis of gene clusters, solvolysis with trifluoroacetic acid
Comments, role: O-deacetylated polysaccharide (DPS)
Related record ID(s): 9724, 9725, 10922
NCBI Taxonomy refs (TaxIDs): 550
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3 aL6dTalp 97.9 71.0 66.7 73.6 68.5 16.8
3,3 aDManp 103.1 67.4 73.6 72.6 74.0 61.5
3 aDManp 102.0 71.1 79.4 66.7 74.6 61.9
2 Ac 175.3 23.6
bDGlcpN 101.8 55.7 80.5 72.9 77.1 62.8
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3 aL6dTalp 5.06 3.89 3.89 3.78 4.51 1.31
3,3 aDManp 5.14 4.26 4.06 3.98 3.82 3.76-3.86
3 aDManp 5.27 4.14 3.85 4.09 3.60 3.83-3.85
2 Ac - 2.05
bDGlcpN 4.52 3.76 3.73 3.38 3.45 3.57-3.98
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3 aL6dTalp 97.9/5.06 71.0/3.89 66.7/3.89 73.6/3.78 68.5/4.51 16.8/1.31
3,3 aDManp 103.1/5.14 67.4/4.26 73.6/4.06 72.6/3.98 74.0/3.82 61.5/3.76-3.86
3 aDManp 102.0/5.27 71.1/4.14 79.4/3.85 66.7/4.09 74.6/3.60 61.9/3.83-3.85
2 Ac 23.6/2.05
bDGlcpN 101.8/4.52 55.7/3.76 80.5/3.73 72.9/3.38 77.1/3.45 62.8/3.57-3.98
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3 | aL6dTalp | 5.06 | 3.89 | 3.89 | 3.78 | 4.51 | 1.31 |
| 3,3 | aDManp | 5.14 | 4.26 | 4.06 | 3.98 | 3.82 | 3.76
3.86 |
| 3 | aDManp | 5.27 | 4.14 | 3.85 | 4.09 | 3.60 | 3.83
3.85 |
| 2 | Ac |
| 2.05 | |
| | bDGlcpN | 4.52 | 3.76 | 3.73 | 3.38 | 3.45 | 3.57
3.98 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3 | aL6dTalp | 97.9 | 71.0 | 66.7 | 73.6 | 68.5 | 16.8 |
| 3,3 | aDManp | 103.1 | 67.4 | 73.6 | 72.6 | 74.0 | 61.5 |
| 3 | aDManp | 102.0 | 71.1 | 79.4 | 66.7 | 74.6 | 61.9 |
| 2 | Ac | 175.3 | 23.6 | |
| | bDGlcpN | 101.8 | 55.7 | 80.5 | 72.9 | 77.1 | 62.8 |
|
There is only one chemically distinct structure:
report error
Found 1 record.
Displayed record 1
