Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: bloody diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ];
hemolytic-uremic syndrome (HUS) [ICD11:
3A21.2 ];
infection due to Escherichia coli [ICD11:
XN6P4 ]
NCBI PubMed ID: 30864133Publication DOI: 10.1007/978-1-4939-9154-9_15Journal NLM ID: 9214969Publisher: Springer
Correspondence: brockhau
queensu.ca
Institutions: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada
Escherichia coli serotype O104:H4 (ECO104) is a potent intestinal pathogen that causes severe bloody diarrhea and hemolytic-uremic syndrome. The O antigenic polysaccharides of ECO104 consist of repeating units with the structure [4Gal α1-4 Neu5,7,9Ac3 α2-3 Gal β1-3 GalNAc β1-]n. These repeating units are assembled sequentially by specific glycosyltransferases on a diphosphate-undecaprenol intermediate. Internal structures include mimics of the human T and sialyl-T antigen. This protocol describes the in vitro synthesis of the repeating unit by β1,3-Gal-transferase WbwC, α2,3-sialyltransferase WbwA, and α1,4-Gal-transferase WbwB. All of these enzymes require acceptor substrates based on GalNAc-diphosphate-lipid. These methods are applicable for the assembly of bacterial polysaccharides of gram-negative bacteria that require sugar-diphosphate intermediates and are a basis for vaccine synthesis.
repeating unit, O antigen, glycosyltransferases, Assays, Escherichia coli O104, In vitro synthesis
Structure type: suggested polymer biological repeating unit
Location inside paper: abstract, p.188, p.189, fig.1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_146100,IEDB_147450,IEDB_149174,IEDB_150933,IEDB_151528,IEDB_190606,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_195,SB_23,SB_24,SB_25,SB_39,SB_68,SB_7,SB_70,SB_8,SB_84,SB_88,SB_97
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, glycosyltransferase assays, ESI-MS, Western blotting, NMR-1D, genetic methods, biochemical methods, radioactivity measurement, HPLC
Comments, role: protocol;
Related record ID(s): 979
NCBI Taxonomy refs (TaxIDs): 2072453Reference(s) to other database(s): GTC:G18884UL, GlycomeDB:
3545
Show glycosyltransferases
There is only one chemically distinct structure:
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