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1. (Article ID: 10)
Beynon LM, Cox AD, Taylor CJ, Wilkinson SG, Perry MB
Characterization of a lipopolysaccharide containing two different trisaccharide repeating units from Burkholderia cepacia serotype E (O2)
Carbohydrate Research 272 (1995)
231-239
The O antigen of the lipopolysaccharide of Burkholderia cepacia serotype E (O2) was shown by a combination of methylation analysis, partial hydrolysis, NMR, and mass spectrometric methods to be a high molecular weight polysaccharide composed of two different trisaccharide repeating units in the ratio 2:1. The major trisaccharide component is composed of two α-D-mannopyranosyl and one β-D-galactopyranosyl residues with the structure, [→2)-α-D-Manp-(1→2)-α-D-Manp-(1→4)-β-D-Galp-(1→]n The minor trisaccharide component is a D-mannan composed of two α- and one β-D-mannopyranosyl residues with the structure, [→2)-α-D-Manp-(1→2)-α-D-Manp-(1→3)-β-D-Manp-(1→]n
Lipopolysaccharide, LPS, structure, characterization, serotype, methylation analysis, 2D NMR (COSY, NOESY) experiments, GCL-MC of alditol acetates, FASBMS, Burkholderia, Burkholderia cepacia, D-mannose, O-antigen, repeating unit, trisaccharide
NCBI PubMed ID: 7497480Publication DOI: 10.1016/0008-6215(95)00095-bJournal NLM ID: 0043535Publisher: Elsevier
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, FAB-MS, partial acid hydrolysis
The publication contains the following compound(s):
- Compound ID: 26
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Reference(s) to other database(s): GTC:G37099RV, GlycomeDB:
25434
- Compound ID: 27
Structure type: polymer chemical repeating unit
Trivial name: mannan
Compound class: O-polysaccharide, O-antigen
Reference(s) to other database(s): GTC:G40440IC, GlycomeDB:
27903
- Compound ID: 28
Structure type: oligomer
Reference(s) to other database(s): GTC:G26595GF, GlycomeDB:
25463
- Compound ID: 29
Structure type: oligomer
Reference(s) to other database(s): GTC:G89329UM, GlycomeDB:
25464
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