Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperNCBI PubMed ID: 30093031Publication DOI: 10.1016/j.carbpol.2018.06.116Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: zzj
tju.edu.cn
Institutions: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
An exopolysaccharide (EPS) was produced by Leuconostoc pseudomesenteroides DRP-5 isolated from homemade wine. The EPS was obtained with ethanol extraction, which was further purified by chromatography of Sephadex G-100 to get a purified fraction. The monosaccharide composition of the EPS was glucose, and its molecular weight (Mw) was 6.23x10(6) Da, as determined by gas chromatography (GC) and high-performance size-exclusion chromatography (HPSEC). Fourier transform infrared spectra (FT-IR) and nuclear magnetic resonance spectra (NMR) showed that the EPS was a linear glucan with α-(1→6)-linked glucosidic bonds. The water holding capacity (WHC), water solubility index (WSI) and emulsifying activity (EA) of DRP-5 EPS were 296.76+/-18.93%, 98.62+/-3.57% and 87.22+/-2.18%, respectively. DRP-5 EPS have a higher degradation temperature of 278.36 degrees C, suggesting high thermal stability of the EPS. Also, DRP-5 EPS was found to have moderate 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, superoxide anion radical, 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical, Fe(2+) scavenging activities and reducing power. All these characteristics suggest that DRP-5 EPS might have potential applications in the pharmaceutical, cosmetic and food industries.
characterization, structural analysis, purification, Antioxidant activity, Leuconostoc pseudomesenteroides dextran
Structure type: structural motif or average structure ; 6230000
Location inside paper: abstract, p.532
Trivial name: dextran
Compound class: EPS
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_158538,IEDB_983931,SB_192
Methods: gel filtration, 13C NMR, 1H NMR, GC, FTIR, composition analysis, HPSEC, statistical analysis, SEM, emulsifying activity, thermodynamic stability analysis, antioxidant activity
NCBI Taxonomy refs (TaxIDs): 33968Reference(s) to other database(s): GTC:G69605LY
Show glycosyltransferases
1H NMR data: present in publication
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13C NMR data: present in publication
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There is only one chemically distinct structure:
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 33710435Publication DOI: 10.1186/s43141-021-00137-yJournal NLM ID: 101317150Publisher: J Genet Eng Biotechnol
Correspondence: kodalividyaprabhakar
gmail.com
Institutions: Department of Biotechnology, Vikrama Simhapuri University, Kakutur, Nellore, A.P-524320, India, Department of Biotechnology, VFSTR University, Vadlamudi, Guntur, A.P-522213, India, SRR and CVR Government Degree College, Machavaram, Vijayawada, A.P-520010, India
Background: Extracellular polysaccharides (ECPs) produced by biofilm-producing marine bacterium have great applications in biotechnology, pharmaceutical, food engineering, bioremediation, and bio-hydrometallurgy industries. The ECP-producing strain was identified as Acinetobacter indicus M6 species by 16S rDNA analysis. The polymer produced by the isolate was quantified and purified and chemically analyzed, and antioxidant activities have been studied. The face-centered central composite design (FCCCD) was used to design the model. Results: The results have clearly shown that the ECP was found to be endowed with significant antioxidative activities. The ECP showed 59% of hydroxyl radical scavenging activity at a concentration of 500 μg/mL, superoxide radical scavenging activity (72.4%) at a concentration of 300 μg/mL, and DPPH˙ radical scavenging activity (72.2%) at a concentration of 500 μg/mL, respectively. Further, HPLC and GC-MS results showed that the isolated ECP was a heteropolymer composed of glucose as a major monomer, and mannose and glucosamine were minor monomers. Furthermore, the production of ECP by Acinetobacter indicus M6 was increased through optimization of nutritional variables, namely, glucose, yeast extract, and MgSO4 by "Response Surface Methodology". Moreover the production of ECP reached to 2.21 g/L after the optimization of nutritional variables. The designed model is statistically significant and is indicated by the R2 value of 0.99. The optimized medium improved the production of ECP and is two folds higher in comparison with the basal medium. Conclusions: Acinetobacter indicus M6 bacterium produces a novel and unique extracellular heteropolysaccharide with highly efficient antioxidant activity. GC-MS analyses elucidated the presence of quite uncommon (1→4)-linked glucose, (1→4)-linked mannose, and (→4)-GlcN-(1→) glycosidic linkages in the backbone. The optimized medium improved the production of ECP and is two folds higher in comparison with the basal medium. The newly optimized medium could be used as a promising alternative for the overproduction of ECP.
extracellular polysaccharide, Antioxidant activity, response surface methodology, Monosaccharide composition
Structure type: structural motif or average structure
Location inside paper: Fig. 7
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
Methods: methylation, GC-MS, acid hydrolysis, UV, RP-HPLC, compositional analysis, radical scavenging assay, antioxidant activity
Comments, role: the probable structure of the EPS
NCBI Taxonomy refs (TaxIDs): 756892
Show glycosyltransferases
There is only one chemically distinct structure:
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