Found 1 publication. Displayed publication 1
Expand all publications       Show all as text (SweetDB notation)
1. (Article ID: 1010)
 
Morath S, Geyer A, Hartung T
Structure-function relationship of cytokine induction by lipoteichoic acid from Staphylococcus aureus
Journal of Experimental Medicine 193(3) (2001) 393-397
 

Lipoteichoic acids (LTAs) have been proposed as putative Gram-positive immunostimulatory counterparts to Gram-negative lipopolysaccharides. However, LTA from Staphylococcus aureus, the clinically most frequent Gram-positive pathogen, was inactive after purification. Here, a novel isolation procedure to prepare pure (>99%) biologically active LTA, allowing the first structural analysis by nuclear magnetic resonance and mass spectrometry, is described. A comparison with LTA purified by standard techniques revealed that alanine substituents are lost during standard purification, resulting in attenuated cytokine induction activity. In line with this finding, hydrolysis of alanine substituents of active LTA decimated cytokine induction. LTA represents a major immunostimulatory component of S. aureus.

Magnetic Resonance Spectroscopy, immunity, Staphylococcus aureus, natural, lipoteichoic acid, tumor necrosis factor, gram-positive bacteria, isolation and purification

The publication contains the following compound(s):
 

Expand this publication

Resort publications by:

New query Export IDs Home Help

Execution: 1 sec