Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
NCBI PubMed ID: 30698425Publication DOI: 10.1021/jacs.8b10940Journal NLM ID: 7503056Publisher: American Chemical Society
Correspondence: gaozhizeng
mail.sysu.edu.cn; withers
chem.ubc.ca
Institutions: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada, Department of Chemistry and Alberta Glycomics Centre, University of Alberta, Edmonton, AB, Canada, Department of Chemistry, University of British Columbia, Vancouver, British Columbia V6T 1Z1, Canada, Department of Biochemistry and Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z1, Canada
Bacterial capsular polysaccharides are important virulence factors. Capsular polysaccharides from several important Gram-negative pathogens share a conserved glycolipid terminus containing 3-deoxy-β-d-manno-oct-2-ulosonic acid (β-Kdo). The β-Kdo glycosyltransferases responsible for synthesis of this conserved glycolipid belong to a new family of glycosyltransferases that shares little homology with other such enzymes, thereby representing an attractive antivirulence target. Here, we report the development of a fluorescence polarization-based, high-throughput screening assay (FP-tag) for β-Kdo glycosyltransferases, and use it to identify a class of marine natural products as lead inhibitors. This 'FP-tag' assay should be readily adaptable to high-throughput screens of other glycosyltransferases.
capsular polysaccharide, glycosyltransferase, inhibitor, Glycomics, high-throughput screening assay (FP-tag)
Structure type: fragment of a bigger structure
Location inside paper: p.2202, fig.2, CPS
Compound class: CPS
Methods: 13C NMR, 1H NMR, glycosyltransferase assays, TLC, ESI-MS, chemical synthesis, HPLC, UV, enzymatic synthesis, conjugation, biotinylation, fluorescence labeling, fluorescent polarization measurements, screening procedures
Biosynthesis and genetic data: KpsC β-Kdo glycosyltransferase
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G02109KF
Show glycosyltransferases
There is only one chemically distinct structure:
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