Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Shigella boydii [ICD11:
XN8RN 
]
NCBI PubMed ID: 15774901Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, School of Molecular and Microbial Biosciences (G08), University of Sydney, Sydney, New South Wales, Australia, TEDA School of Biology Sciences and Biotechnology, Nankai University, TEDA, Tianjin State Laboratory of Microbial Functional Genomics, TEDA College, Nankai University, Tianjin, State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China
Comparison of the O antigens of Shigella boydii types 10 and 6 by chemical analysis and nuclear magnetic resonance spectroscopy showed that their structures are similar, with the only difference being the presence or absence of d-ribofuranose, which is the immunodominant sugar in S. boydii type 10. In S. boydii type 6, a residue previously reported as α-D-GlcpA, was shown to be β-D-GlcpA as in S. boydii type 10. S. boydii types 10 and 6 are reported not to cross-react serologically, and the role of d-ribofuranose in the specificity of S. boydii was confirmed by making a mutant of type 10 that lacked d-ribofuranose. However, S. boydii type 11, which has a d-ribofuranose but with different linkage does show cross-reaction with type 10. The O-antigen gene loci of S. boydii types 10 and 6 were shown to be virtually identical except that orf8 (wbaM), which was confirmed as the ribofuranosyltransferase gene, is interrupted by IS629 in type 6. Therefore, it is proposed that the O-antigen gene cluster of S. boydii type 6 was derived from type 10 by an IS element insertion.
genetic, structure, gene, structural, characterization, O-antigen, O antigen, type, O antigens, O-antigens, Shigella, function, clone, Shigella boydii
Structure type: polymer chemical repeating unit
Location inside paper: p.2552
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_130701,IEDB_136104,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_140630,IEDB_141794,IEDB_143632,IEDB_144983,IEDB_149136,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_423153,IEDB_983930,SB_136,SB_196,SB_21,SB_44,SB_67,SB_7,SB_72
Methods: methylation, NMR, sugar analysis
Biosynthesis and genetic data: genetic data
Related record ID(s): 10331, 23051
NCBI Taxonomy refs (TaxIDs): 621Reference(s) to other database(s): GTC:G38242ZP, GlycomeDB:
27644
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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