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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_114701,IEDB_136045,IEDB_1394181,IEDB_142489,IEDB_144562,IEDB_152214,IEDB_167188,IEDB_174332,IEDB_174333,SB_86

• Article ID: 4279
  Boyko AS, Dmitrenok AS, Fedonenko YP, Zdorovenko EL, Konnova SA, Knirel YA, Ignatov VV "Structural analysis of the O-polysaccharide of the lipopolysaccharide from Azospirillum brasilense Jm6B2 containing 3-O-methyl-D-rhamnose (D-acofriose)" - Carbohydrate Research 355 (2012) 92-95
  

  Two types of neutral O-polysaccharides were obtained by mild acid degradation of the lipopolysaccharide isolated by phenol-water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense Jm6B2. The following structure of the major O-polysaccharide was established by composition and methylation (ethylation) analyses, Smith degradation, and 1D and 2D (1)H and (13)C NMR spectroscopy: [structure: see text] where a non-stoichiometric (~60%) 3-O-methylation of D-rhamnose is indicated by italics.

  Lipopolysaccharide, O-antigen, Azospirillum brasilense, bacterial polysaccharide structure, D-Acofriose

  NCBI PubMed ID: 22575749
  Publication DOI: 10.1016/j.carres.2012.04.006
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: room308@ibppm.sgu.ru
  Institutions: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia
  Methods: 13C NMR, 1H NMR, methylation, NMR-2D, chemical analysis, GLC, Smith degradation, NMR-1D
  
   
  
  Azospirillum brasilense Jm6B2
  CSDB ID 27122 (all data & tools)
• Article ID: 4616
  Sigida EN, Fedonenko YP, Zdorovenko EL, Konnova SA, Shashkov AS, Ignatov VV, Knirel YA "Structure of repeating units of a polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense SR80" - Carbohydrate Research 371 (2013) 40-44
  

  A high-molecular mass polysaccharide fraction was obtained by mild acid hydrolysis of the lipopolysaccharide of diazotrophic rhizobacterium Azospirillum brasilense SR80 followed by GPC on Sephadex G-50 Superfine. Studies by composition and methylation analyses, Smith degradation, and 1D and 2D (1)H and (13)C NMR spectroscopy demonstrated the presence of two structurally distinct repeating units having the following structures: It seems likely, although not proved, that these are repeats of two distinct polysaccharides rather than they build blocks within the same polysaccharide chain. The former structure is new, whereas the latter is closely related to the O-polysaccharide structure of A. brasilense Jm6B2 established earlier, which differs in partial (~60%) 3-O-methylation of d-rhamnose only [Boiko, A. S.; Dmitrenok, A. S.; Fedonenko, Yu. P.; Zdorovenko, E. L.; Konnova, S. A.; Knirel, Y. A.; Ignatov, V. V. Carbohydr. Res.2012, 355, 92-95].

  Lipopolysaccharide, Azospirillum brasilense, O-Polysaccharide structure

  NCBI PubMed ID: 23500958
  Publication DOI: 10.1016/j.carres.2013.01.005
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: E.L. Zdorovenko
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia
  Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, Smith degradation, composition analysis, GPC
  
   
  
  Azospirillum brasilense SR80, Azospirillum brasilense Jm6B2
  CSDB ID 29762 (all data & tools)
• Article ID: 4617
  Sigida EN, Fedonenko YP, Shashkov AS, Zdorovenko EL, Konnova SA, Ignatov VV, Knirel YA "Structural studies of the O-specific polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense type strain Sp7" - Carbohydrate Research 380 (2013) 76-80
  

  Lipopolysaccharide was obtained by phenol-water extraction from dried bacterial cells of Azospirillum brasilense type strain Sp7. Mild acid hydrolysis of the lipopolysaccharide followed by GPC on Sephadex G-50 resulted in a polysaccharide mixture, which was studied by composition and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy. The following polysaccharide structures were established, where italics indicate a non-stoichiometric (~40%) 2-O-methylation of l-rhamnose.

  Lipopolysaccharide, Azospirillum brasilense, bacterial polysaccharide structure

  NCBI PubMed ID: 23978662
  Publication DOI: 10.1016/j.carres.2013.07.013
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: EA: E.L. Zdorovenko ; evelina@ioc.ac.ru
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia
  Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, GLC, mild acid hydrolysis, Smith degradation, composition analysis, GPC
  
   
  
  Azospirillum brasilense Sp17 (ATCC 29145)
  CSDB ID 29767 (all data & tools)
• Article ID: 4724
  Sigida EN, Fedorova EE, Zdorovenko EL, Burygin GL, Konnova SA, Ignatov VV "Characterization of the lipopolysaccharides of serogroup II Azospirillum strains" - Mikrobiologiia = Microbiology [Russian] 83(4) (2014) 326-334
  

  Lipopolysaccharides of six Azospirillum brasilense strains (SR50, SR80, SR88, SR109, SR111, SR115) and A. lipoferum SR 42 isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and referred to serogroup II by serological analysis were studied. In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by a predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids. Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios. A high degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure of their O antigens.

  lipopolysaccharides, Azospirillum, structure of the Ospecific polysaccharide, serological investigation

  Publication DOI: 10.1134/S0026261714040158
  Journal NLM ID: 0376652
  Publisher: Moskva: Izdatelstvo Nauka
  Correspondence: Sigida EN
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia, Chernyshevsky Saratov State University, Russia
  Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, ELISA, GLC, mild acid hydrolysis, Western blotting, serological methods, GPC
  
   
  
  Azospirillum brasilense SR42, Azospirillum brasilense SR88, Azospirillum brasilense SR 109, Azospirillum brasilense SR111, Azospirillum brasilense SR115, Azospirillum brasilense SR80, Azospirillum brasilense SR50, Azospirillum brasilense Sp
  CSDB ID 30463 (all data & tools)
• Article ID: 4844
  Fedonenko YP, Sigida EN, Konnova SA, Ignatov VV "Structure and serology of O-antigens of nitrogen-fixing rhizobacteria of the genus Azospirillum" - Russian Chemical Bulletin = Izvestiia Akademii nauk. Seriia khimicheskaia 64(5) (2015) 1024-1031
  

  The review presents a discussion of the published data of 2002 to 2014 dealing with structural studies of O-specific polysaccharides, representatives of the Azospirillum diazotrophic rhizobacteria, summarizing the results of a series of authors? studies. Some general structures incorporated in O-specific polysaccharides responsible for classification of bacterial cultures into serogroups on the basis of immunochemical studies were characterized. The structures of O-specific polysaccharides of seven azospirillum strains identical to those of previously studied strains are presented in this review for the first time.

  lipopolysaccharides, O-specific polysaccharides, Structures, Azospirillum, O-antigenic determinants

  Publication DOI: 10.1007/s11172-015-0971-x
  Journal NLM ID: 100912060
  Publisher: New York: Consultants Bureau
  Correspondence: si_elena@mail.ru
  Institutions: Institute of Biochemistry and Physiology of Plants and Microorganisms Russian Academy of Sciences, 13 prosp. Entuziastov, 410049 Saratov, Russian Federation
  
   
  
  Azospirillum brasilense SR88, Azospirillum brasilense SR109, Azospirillum brasilense SR111, Azospirillum brasilense SR115, Azospirillum brasilense SR80, Azospirillum brasilense SR50, Azospirillum lipoferum SR42, Azospirillum brasilense Sp7
  CSDB ID 11167 (all data & tools)