Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 15721335Publication DOI: 10.1016/j.carres.2005.01.001Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: G. Widmalm <gw
organ.su.se>
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden, Department of Chemistry and Physiological Sciences, Universidad Nacional Autonoma de Nicaragua, UNAN-Leon, Nicaragua, Department of Laboratory Medicine, Division of Clinical Bacteriology, Karolinska Institutet, Karolinska University Hospita, Huddinge, Stockholm, Sweden, The International Escherichia and Klebsiella Centre (WHO), Artillerivej 5, DK-2300 Copenhagen S, Denmark
The structure of the O-antigen polysaccharide (PS) from the enteroaggregative Escherichia coli strain 180/C3 has been determined. Sugar and methylation analysis together with (1)H and (13)C NMR spectroscopy were the main methods used. The PS is composed of tetrasaccharide repeating units with the following structure:Analysis of NMR data indicates that the presented sequence of sugar residues also represents the biological repeating unit of the O-chain. The structure is closely related to that of O-antigen polysaccharide from E. coli O5 and partially to that of E. coli O65. The difference between the O-antigen from the 180/C3 strain and that of E. coli O5 is the linkage to the d-Quip3NAc residue, which in the latter strain is 4-O-substituted. The E. coli O65 O-antigen contains as part of its linear pentasaccharide repeating unit a similar structural element, namely →4)-β-D-GalpA-(1→3)-α-D-GlcpNAc-(1→2)-β-D-Quip3NAc-(1→, thereby indicating that a common epitope could be present for the two polysaccharides. Monospecific anti-E. coli O5 rabbit serum did not distinguish between the two positional isomeric structures neither in slide agglutination nor in an indirect enzyme immunoassay. The anti-O65 serum did react with both the 180/C3 and O5 LPS showing a partial cross-reactivity
Lipopolysaccharide, NMR, LPS, structure, common, tetrasaccharide, chemistry, clinical, strain, structural, polysaccharide, methylation analysis, O-antigen, repeating unit, analysis, polysaccharides, O antigen, Escherichia, Escherichia coli, O-antigenic, O-antigenic polysaccharide, epitope, NMR spectroscopy, serology, biological, linkage, sugar, methylation, medicine, spectroscopy, method, elucidation, immunochemical, sequence, enzyme, O-chain, difference, pentasaccharide, methods, linear, relationship, serum, cross-reactivity, crossreactivity, bacteriology, enzyme immunoassay, immunoassay, indirect enzyme immunoassay, biological repeating unit, Enteroaggregative, rabbit, physiological, agglutination
Structure type: suggested polymer biological repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_151531,IEDB_885822
Methods: methylation, NMR, sugar analysis
Biological activity: serological data
Comments, role: structure was ambiguously pasted from the original publication; validated basing on ID 4043; biological repeat frame was based on GNE gene presence
Related record ID(s): 4043, 10174, 10338, 20698
NCBI Taxonomy refs (TaxIDs): 2162912Reference(s) to other database(s): GTC:G86652HR, GlycomeDB:
28132
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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