Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
NCBI PubMed ID: 15961298Publication DOI: 10.1016/j.femsim.2005.05.003Journal NLM ID: 9315554Publisher: Elsevier
Correspondence: katzenel
immuno.iitd.pan.wroc.pl
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
Earlier, the structures of the O-chain polysaccharides of the lipopolysaccharides (LPS) of a number of Hafnia alvei strains have been established. However, it remained unknown, which is the first and the last monosaccharide of the O-chain. This is defined by the structure of the so-called biological repeating unit (O-unit), which is pre-assembled and then polymerised in the course of biosynthesis of bacterial polysaccharides by the Wzy-dependent pathway. Now we report on the structures of the O-units in 10 H. alvei strains. The LPS were cleaved by mild acid hydrolysis and oligosaccharide fractions IIIa and IIIb were isolated by gel chromatography subsequently on Sephadex G-50 and BioGel P-2 and studied by methylation analysis and NMR spectroscopy. Fraction IIIb was found to represent the core oligosaccharide containing a terminal upstream α-D-Glc-(1→3)-α-D-Glc or α-D-Gal-(1→3)-α-D-Glc disaccharide in the outer region that is typical of H. alvei. Fraction IIIa consists of the LPS core with one O-unit linked by a 3-substituted β-D-GalNAc residue (in strains PCM 1189 and PCM 1546) or a 3-substituted β-D-GlcNAc residue (in the other strains studied). In most strains examined the β-configuration of the d-GlcNAc linkage in the first O-unit attached to the core is the same and in some strains is opposite to that found in the interior O-units of the O-chain polysaccharide. Various monosaccharides, including d-Glc, d-Gal, d-GlcA and acyl derivatives of 3-amino-3,6-dideoxy-d-glucose or 4-amino-4,6-dideoxy-d-glucose, occupy the non-reducing end of the O-unit.
Lipopolysaccharide, Hafnia alvei, O-antigen structure, biological repeating unit
Structure type: polymer biological repeating unit
Location inside paper: p.275
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_221845,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_21,SB_6,SB_7,SB_88
Methods: methylation, NMR
Biological activity: serological data
Comments, role: published data
Related record ID(s): 4937, 10381, 11682, 20649, 23242, 26890, 27435, 28337, 30360, 108742, 115996
NCBI Taxonomy refs (TaxIDs): 569Reference(s) to other database(s): GTC:G92807QP, GlycomeDB:
3591
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There is only one chemically distinct structure:
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