Fucose-containing oligosaccharides (FCOs) have important applications in the food, medicine, and cosmetics industries owing to their unique biological activities. The degradation of microbial fucose-containing exopolysaccharide (FcEPS) is a promising strategy for obtaining FCOs, and bacteriophage-borne glycanase is a useful tool for degrading FcEPS. Here, we aimed to obtain FCOs using bacteriophage-borne glycanase to depolymerize FcEPS from Enterobacter sakazakii. The FcEPS was mainly composed of l-fucose (42.72 %), d-galactose (20.59 %), and d-glucose (21.81 %). Based on the results of nuclear magnetic resonance and mass spectrometry, the obtained FCOs were disaccharide fragments with backbones of β-d-Glcp-(1→4)-β-l-Fucp and α-d-Galp-(1→3)-β-l-Fucp, respectively. So far, few studies of disaccharides prepared from FcEPS have been reported. This study demonstrated that the FcEPS of E. sakazakii was a reliable fucose-containing disaccharide source and that bacteriophage-borne glycanase was an effective degradation tool for obtaining FCOs fragments from FcEPS.

fucose, Enterobacter sakazakii, bacteriophage-borne glycanase, fucose-containing disaccharide, microbial exopolysaccharide

13C NMR data:
missing...
1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
4	bDGlcp	4.634	3.471	3.665	4.005	3.551	3.671-3.790
	bLFucp	4.532	3.472	3.541	3.787	3.905	1.377

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

C[C@@H]1O[C@H](O)[C@@H](O)[C@H](O)[C@@H]1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O

326.298 g/mol (C₁₂H₂₂O₁₀)

Fucose-containing oligosaccharides (FCOs) have important applications in the food, medicine, and cosmetics industries owing to their unique biological activities. The degradation of microbial fucose-containing exopolysaccharide (FcEPS) is a promising strategy for obtaining FCOs, and bacteriophage-borne glycanase is a useful tool for degrading FcEPS. Here, we aimed to obtain FCOs using bacteriophage-borne glycanase to depolymerize FcEPS from Enterobacter sakazakii. The FcEPS was mainly composed of l-fucose (42.72 %), d-galactose (20.59 %), and d-glucose (21.81 %). Based on the results of nuclear magnetic resonance and mass spectrometry, the obtained FCOs were disaccharide fragments with backbones of β-d-Glcp-(1→4)-β-l-Fucp and α-d-Galp-(1→3)-β-l-Fucp, respectively. So far, few studies of disaccharides prepared from FcEPS have been reported. This study demonstrated that the FcEPS of E. sakazakii was a reliable fucose-containing disaccharide source and that bacteriophage-borne glycanase was an effective degradation tool for obtaining FCOs fragments from FcEPS.

fucose, Enterobacter sakazakii, bacteriophage-borne glycanase, fucose-containing disaccharide, microbial exopolysaccharide

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
3	aDGalp	103.990	73.346	70.147	70.934	72.422	62.587
	bLFucp	94.321	70.488	77.929	71.546	68.410	17.250


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
3	aDGalp	5.260	3.824	4.063	3.871	3.488	3.550-3.762
	bLFucp	4.324	3.778	3.487	3.667	3.837	1.331


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
3	aDGalp	103.990/5.260	73.346/3.824	70.147/4.063	70.934/3.871	72.422/3.488	62.587/3.550-3.762
	bLFucp	94.321/4.324	70.488/3.778	77.929/3.487	71.546/3.667	68.410/3.837	17.250/1.331

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

C[C@@H]1O[C@H](O)[C@@H](O)[C@H](O[C@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@@H]1O

326.298 g/mol (C₁₂H₂₂O₁₀)