Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 7030219Journal NLM ID: 0372430Structure type: oligomer
Compound class: mannan, trisaccharide
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: gel filtration, TLC, GC, acid degradation, methylation analysis, precipitin reaction, alkali degradation, Cetavlon treatment, modified Fehling solution method
Comments, role: degradation product; structure not clearly assigned to a taxon
Related record ID(s): 130520, 140208
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G25559RS, CCSD:
8676, CBank-STR:6340
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 2643567Journal NLM ID: 0246127Publisher: American Society for Microbiology
Institutions: Department of Microbiology, School of Medicine, Georgetown University, Washington, D.C. 20007
We previously reported the isolation of two cerulenin-resistant mutant strains of Candida albicans 4918 that differ in adherence properties and are less virulent than the parental strain. In addition, biochemical characterization demonstrated significant differences in both protein and polysaccharide composition of cell wall material between the mutant and wild-type strains. These observations prompted studies concerning the chemical structure of mannans in these strains. After extraction and subsequent purification by ion-exchange chromatography, mannan fractions were subjected to either mild acid hydrolysis, alkali hydrolysis, or acetylation followed by acetolysis. Acid- and alkali-modified mannans were studied by proton magnetic resonance spectroscopy, and released products were analyzed by high-performance liquid chromatography on an Aminex HPX-42A column. The results demonstrated quantitative and qualitative differences between mannooligosaccharides of the wild-type and mutant strains in the identity of released oligosaccharides as well as in linkage of the oligosaccharides to the protein backbone.
Structure type: homopolymer ; n=1-5
Location inside paper: p. 417
Contained glycoepitopes: IEDB_137485,IEDB_140116,IEDB_144983,IEDB_152206,IEDB_76920,IEDB_983930,SB_44,SB_72
Methods: acid hydrolysis, HPLC, acetylation, acid degradation, acetolysis, gel-filtration, precipitation, thin-layer chromatography, PMR, alkali degradation
Comments, role: imported from CCSD but clear structure is absent from the publication
NCBI Taxonomy refs (TaxIDs): 5476Reference(s) to other database(s): GTC:G40314ZR, CCSD:
21079, CBank-STR:10741
Show glycosyltransferases
There is only one chemically distinct structure: