From the roots of Bupleurum wenchuanense 14 derivatives of saikosaponin were isolated and identified as 2″-O-β-d-xylopyranosylsaikosaponin b2, 3″,6″-O,O-diacetylsaikosaponin b2, 2″-O-β-d-glucopyranosylsaikosaponin b2, saikosaponin b2, 6″-O-acetylsaikosaponin b2, saikosaponin d, 2″-O-acetylsaikosaponin d, 3″-O-acetylsaikosaponin d, 6″-O-acetylsaikosaponin d, 16-epichikusaikoside, prosaikogenin G, saikosaponin a, 2″-O-acetylsaikosaponin a and 3″-O-acetylsaikosaponin a. The first two compounds are new derivatives of saikosaponin and this is the first isolation of prosaikogenin G from a plant. Their complete 1H and 13C NMR assignments were made by using a combination of 2D NMR techniques (DQF-COSY, HOHAHA, ROESY, HETCOR, HMQC and HMBC). Some of the compounds showed cytotoxic activity against the P-388 cell line.

cytotoxicity, Umbelliferae, Bupleurum wenchuanense, saikosaponin glucosides, 2″-O-β-d-xylopyranosylsaikosaponin b2, 3″, 6″-O, O-diacetylsaikosaponin b2, 2″-O-β-d-glucopyranosylsaikosaponin b2, saikosaponin b2, 6″-O-acetylsaikosaponin b2, saikosaponin d, 2″-O-acetylsaikosaponin d, 3″-O-acetylsaikosaponin d, 6″-O-acetylsaikosaponin d, 16-epichikusaikoside, prosaikogenin G, saikosaponin a, 2″-O-acetylsaikosaponin a, 3″-O-acetylsaikosaponin a

• Compound ID: 23791
  

  b-D-Glcp-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin F = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=C[C@]45[C@]6([H])CC(C)(C)CC[C@@](CO5)6{16}[C@@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: saikosaponin a, saikosaponin-a
  Compound class: saponin glycoside, glycoside, triterpenoid glycoside
  Reference(s) to other database(s): CCSD:27760, CBank-STR:3093
  
   
  
  Bupleurum wenchuanense
  CSDB ID 295994 (all data & tools)
• Compound ID: 23792
  

  b-D-Glcp-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin G = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=C[C@]45[C@]6([H])CC(C)(C)CC[C@@](CO5)6{16}[C@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: saikosaponin d, saikosaponin-d
  Compound class: saponin glycoside, glycoside, triterpenoid glycoside
  Reference(s) to other database(s): CCSD:933, CBank-STR:5893
  
   
  
  Bupleurum wenchuanense
  CSDB ID 245990 (all data & tools)
• Compound ID: 23794
  

  b-D-Glcp-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin D = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=CC4=C5CC(C)(C)CC[C@@]({28}CO)5{16}[C@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: saikosaponin a, saikosaponin b2
  Compound class: saponin glycoside, glycoside, steroid glycoside
  Reference(s) to other database(s): CCSD:26960, CBank-STR:5890
  
   
  
  Bupleurum wenchuanense
  CSDB ID 295988 (all data & tools)
• Compound ID: 24418
  

  b-D-Glcp3Ac-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin G = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=C[C@]45[C@]6([H])CC(C)(C)CC[C@@](CO5)6{16}[C@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: 3″-O-acetylsaikosaponin d
  Compound class: saponin glycoside
  
   
  
  Bupleurum wenchuanense
  CSDB ID 295991 (all data & tools)
• Compound ID: 24420
  

  b-D-Glcp6Ac-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin G = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=C[C@]45[C@]6([H])CC(C)(C)CC[C@@](CO5)6{16}[C@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: 6″-O-acetylsaikosaponin d
  Compound class: saponin glycoside
  
   
  
  Bupleurum wenchuanense
  CSDB ID 295992 (all data & tools)
• Compound ID: 24421
  

  b-D-Glcp3Ac-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin F = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=C[C@]45[C@]6([H])CC(C)(C)CC[C@@](CO5)6{16}[C@@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: 3″-O-acetylsaikosaponin a
  Compound class: saponin glycoside
  
   
  
  Bupleurum wenchuanense
  CSDB ID 295996 (all data & tools)
• Compound ID: 24422
  

  b-D-Glcp2Ac-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin F = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=C[C@]45[C@]6([H])CC(C)(C)CC[C@@](CO5)6{16}[C@@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: 2″-O-acetylsaikosaponin a
  Compound class: saponin glycoside
  
   
  
  Bupleurum wenchuanense
  CSDB ID 295995 (all data & tools)
• Compound ID: 24429
  

  b-D-Glcp6Ac-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin D = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=CC4=C5CC(C)(C)CC[C@@]({28}CO)5{16}[C@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: 6″-O-acetylsaikosaponin b2
  Compound class: saponin glycoside
  
   
  
  Bupleurum wenchuanense
  CSDB ID 295989 (all data & tools)
• Compound ID: 25203
  

  b-D-Glcp-(1-2)-b-D-Glcp-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin D = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=CC4=C5CC(C)(C)CC[C@@]({28}CO)5{16}[C@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: 2"-O-β"-D-glucopyranosylsaikosaponin-b2 saikosaponin, 2"-O-β-D-glucopyranosylsaikosaponin-b2
  Compound class: saponin glycoside, glycoside, steroid glycoside
  Reference(s) to other database(s): CCSD:29774, CBank-STR:9531
  
   
  
  Bupleurum wenchuanense
  CSDB ID 145989 (all data & tools)
• Compound ID: 25624
  

  b-D-Xylp-(1-2)-b-D-Glcp-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin D = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=CC4=C5CC(C)(C)CC[C@@]({28}CO)5{16}[C@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer ; 912 [M]+
  C47H76O17
  Trivial name: 2"-O-β-D-xylopyranosylsaikosaponin-b2
  Compound class: saponin glycoside
  Reference(s) to other database(s): CCSD:29773, CBank-STR:9464
  
   
  
  Bupleurum wenchuanense
  CSDB ID 145988 (all data & tools)
• Compound ID: 25625
  

  b-D-Glcp3Ac6Ac-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin D = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=CC4=C5CC(C)(C)CC[C@@]({28}CO)5{16}[C@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  C46H72O15
  Trivial name: 3",6"-O,O-diacetylsaikosaponin b2
  Compound class: saponin glycoside
  
   
  
  Bupleurum wenchuanense
  CSDB ID 245988 (all data & tools)
• Compound ID: 25626
  

  b-D-Xylp-(1-2)-b-D-Glcp-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin G = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=C[C@]45[C@]6([H])CC(C)(C)CC[C@@](CO5)6{16}[C@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: 16-epichikusaikoside
  Compound class: saponin glycoside
  Reference(s) to other database(s): CCSD:29775, CBank-STR:9462
  
   
  
  Bupleurum wenchuanense
  CSDB ID 145990 (all data & tools)
• Compound ID: 25627
  

  b-D-Glcp2Ac-(1-3)-b-D-Fucp-(1-3)-Subst Subst = saikogenin G = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=C[C@]45[C@]6([H])CC(C)(C)CC[C@@](CO5)6{16}[C@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: 2″-O-acetylsaikosaponin d
  Compound class: saponin glycoside
  
   
  
  Bupleurum wenchuanense
  CSDB ID 295990 (all data & tools)
• Compound ID: 25628
  

  b-D-Fucp-(1-3)-Subst Subst = saikogenin G = SMILES C[C@@]1({23}CO){3}[C@@H](O)CC[C@]2(C)[C@@]3([H])C=C[C@]45[C@]6([H])CC(C)(C)CC[C@@](CO5)6{16}[C@H](O)C[C@](C)4[C@@](C)3CC[C@@]12[H]

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: prosaikogenin G
  Compound class: saponin glycoside
  
   
  
  Bupleurum wenchuanense
  CSDB ID 295993 (all data & tools)