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1. (Article ID: 10475)
 
Vietor RJ, Hoffmann RA, Angelino SAGF, Voragen AGJ, Kamerling JP, Vliegenthart JFG
Structures of small oligomers liberated from barley arabinoxylans by endoxylanase from Aspergillus awamori
Carbohydrate Research 254 (1994) 245-255
 

Arabinoxylans consisting of more than 90% of arabinose and xylose were extracted from water-in-soluble cell wall material of dehusked barley grain, and degraded with a purified endo-(1→4)-β-d-xylanase from Aspergillus awamori. Twelve of the oligosaccharide fragments released were isolated by a combination of size-exclusion and anion-exchange chromatography, and their structures were determined by 1H NMR spectroscopy. The oligosaccharides identified consisted of (1→4)-linked β-d-xylopyranosyl residues, some of which were substituted at O-2, at O-3, or at both O-2 and O-3, with α-l-arabinofuranosyl groups. Two new structures, a tetrasaccharide and a pentasaccharide, both containing terminal 2-O-arabinofuranosylxylopyranose as a structural element are described.

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