Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 1536645Journal NLM ID: 2984726RPublisher: London, UK : Published by Portland Press on behalf of the Biochemical Society
Institutions: Departamento de Microbiología Aplicada, Centro de Investigaciones Biológicas (C.S.I.C.), Madrid, Spain
An endo β-(1----5)-galactofuranase from Penicillium oxalicum has been purified 91-fold. The enzyme is a basic glycoprotein with a pI 7.9 and 20% (w/w) carbohydrate content, galactose being the principal sugar. The apparent Mr of the enzyme estimated by denaturing gel electrophoresis was 77,000. The optimum pH was 5.0, and the enzyme was stable over the pH range 4.0-7.5. This enzyme hydrolyses specifically (1----5)-linked β-D-galactofuranose residues in homo- and heterogalactans, but did not hydrolyse o-nitrophenyl galactose and β-(1----5)-galactofuranbiose. Km and Vmax. values were 1.2 mg.ml-1 and 0.55 mumol.h-1 respectively when Eupenicillium crustaceum β-(1----5)-galactofuran was used as substrate. The enzyme showed high affinity for different separation gels and proteins. The enzyme specificity and its mode of action showed that it could be an useful tool for analysing the fine structure of polysaccharides.
Structure type: homopolymer
Trivial name: galactan, PAM-galactan, galactofuranomannan
Compound class: EPS, cell wall polysaccharide, arabinogalactan, galactan
Contained glycoepitopes: IEDB_136095,IEDB_137472,IEDB_149137,IEDB_190606,IEDB_885812
Methods: acid hydrolysis, Smith degradation, PAGE, periodate oxidation, IEF, enzymatic assay, methylation analysis (the Hakomori method)
Related record ID(s): 126019
NCBI Taxonomy refs (TaxIDs): 68832Reference(s) to other database(s): GTC:G92108RF, CCSD:
7498, CBank-STR:2546
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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