To date, the structures of the glycolipids from Lactobacillus plantarum, a commonly used beneficial probiotic, have not been conclusively assigned. Herein, we report for the first time, the full characterisation of the four principal glycolipids of the L. plantarum cell wall using sugar, linkage and FAME analysis, as well as ESI-MS/MS and 1D- and 2D-NMR spectroscopy, and assign the major glycolipids as being: α-D-Glcp-diglyceride, α-D-Galp-(1→2)-α-D-Glcp-diglyceride, β-D-Glcp-(1→6)-α-D-Galp-(1→2)-6-O-acyl-α-D-Glcp-diglyceride and β-D-Glcp-(1→6)-α-D-Galp-(1→2)-α-D-Glcp-diglyceride.
structure elucidation, Lactobacillus, glycolipids, natural products, Lactobacillus plantarum, Glycerides
NCBI PubMed ID: 22683117Publication DOI: 0.1016/j.carres.2012.05.011Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: M.S.M. Timmer
Institutions: School of Chemical and Physical Sciences, Victoria University of Wellington, PO Box 600, Wellington, New Zealand, Industrial Research Limited, PO Box 31-310, Lower Hutt, New Zealand, Malaghan Institute of Medical Research, PO BOX 7060, Wellington, New Zealand
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, sugar analysis, TLC, ESI-MS, ESI-MS/MS, GC, chemical methods, NMR-1D
Found