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1. (Article ID: 10866)
 
Hino K, Yamamoto S, Sano O, Taniguchi Y, Kohno K, Usui M, Fukuda S, Hanzawa H, Haruyama H, Kurimoto M
Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen
Journal of Biochemistry 117 (1995) 289-295
 

The glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen was treated with pepsin and glycopeptidase A to release asparagine-linked oligosaccharides. The reducing ends of the oligosaccharides were aminated with the fluorescent reagent 2-aminopyridine. The oligosaccharide derivatives were purified by gel permeation chromatography and reversed-phase HPLC. Their structures were determined by sequential exoglycosidase digestion and 500 MHz 1H-NMR spectroscopy. Four oligosaccharide structures, A, B, C, and D, were identified as the xylose-containing complex-type. They were present at a molar ratio of 8:1:6:1. By amino acid sequence analyses of the tryptic peptides, Asn-170 and Asn-333 of Cry j I were found to carry asparagine-linked oligosaccharides.

glycoprotein, carbohydrate structure, 1H-NMR, allergen, Cry j I

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2. (Article ID: 10957)
 
Van HL, Kuraishi S, Sakurai N
Aluminum-induced rapid root inhibition and changes in cell-wall components of squash seedlings
Plant Physiology 106 (1994) 971-976
 

Growth of squash (Cucurbita maxima Duch.) roots was significantly inhibited by 1 mM AlCl3 as early as 1 h after the treatment. The growth inhibition was confined to the elongating zone (1-6 mm from the root tip). Chemical analysis of cell-wall polysaccharides from roots revealed that aluminum increased pectin, hemi-cellulose, and cellulose contents after 3 h of treatment. The effect of aluminum on pectin content was found in the elongating zone including the root tip, whereas change in cellulose content was confined to only nonelongating zones. Hemicellulose content increased in all of the regions along the root axis. The increase in the pectin fraction was due to the increases in uronic acids, galactose, and arabinose constituents, whereas hemicellulose content changed due to increases in glucose, xylose, galactose, and arabinose. The results clearly indicate that aluminum rapidly reduced squash root growth by inhibiting cell elongation and altering metabolism of cell-wall polysaccharides in the nonelongating zone as well as in the elongating zone.

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