Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Pseudomonas aeruginosa [ICD11:
XN5L6 
]
The structure was elucidated in this paperNCBI PubMed ID: 33626358Publication DOI: 10.1016/j.celrep.2021.108782Journal NLM ID: 101573691Publisher: Cambridge, MA: Cell Press
Correspondence: parsem
u.washington.edu
Institutions: Department of Microbiology, University of Washington, Seattle, WA 98195, USA, Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH 43210, USA, Department of Microbiology, The Ohio State University, Columbus, OH 43210, USA, Infectious Disease Institute, The Ohio State University, Columbus, OH 43210, USA, Department of Medicine, University of Washington, Seattle, WA 98195, USA
In cystic fibrosis (CF) airways, Pseudomonas aeruginosa forms cellular aggregates called biofilms that are thought to contribute to chronic infection. To form aggregates, P. aeruginosa can use different mechanisms, each with its own pathogenic implications. However, how they form in vivo is controversial and unclear. One mechanism involves a bacterially produced extracellular matrix that holds the aggregates together. Pel and Psl exopolysaccharides are structural and protective components of this matrix. We develop an immunohistochemical method to visualize Pel and Psl in CF sputum. We demonstrate that both exopolysaccharides are expressed in the CF airways and that the morphology of aggregates is consistent with an exopolysaccharide-dependent aggregation mechanism. We reason that the cationic exopolysaccharide Pel may interact with some of the abundant anionic host polymers in sputum. We show that Pel binds extracellular DNA (eDNA) and that this interaction likely impacts current therapies by increasing antimicrobial tolerance and protecting eDNA from digestion.
exopolysaccharide, cystic fibrosis, Biofilm, Psl, Pel, antibiotic susceptibility, bacterial aggregation, extracellular DNA, IHC, Pseudomonas aeruginosa biofilm matrix
Structure type: structural motif or average structure
Location inside paper: Fig. 3A
Trivial name: Pel
Compound class: EPS
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_137340,IEDB_137473,IEDB_141807,IEDB_149155,IEDB_151531
Methods: PCR, DNA techniques, enzymatic digestion, statistical analysis, binding assays, biofilm assays, confocal microscopy, immunohistochemistry (IHC), solid-state NMR
Comments, role: Half of the Pel sugar residues are deacetylated.
NCBI Taxonomy refs (TaxIDs): 287
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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