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1. (Article ID: 10870)
 
Siddiqui HL, Munesada K, Suga T
Biologically active clerodane-type diterpene alcohol and its glycosides from the root-stalks of ferns
Journal of the Chemical Society, Perkin Transactions 1 (1992) 781-785
 

A diterpene alcohol showing a growth inhibition of lettuce was isolated from the root-stalks of Dicranopteris pedata and Gleichenia japonica. Its structure was determined to be (6S,13S)-cleroda-3,14-diene-6,13-diol 1, an aglycone of the two glycosides previously isolated from G. japonica; the chirality at both C-6 and C-13 was assigned as S by application of the β-D-glucosylation-shift rule and by measurement of 13C NMR chemical shifts, respectively. Further, two glycosides showing growth inhibition were also isolated from D. pedata and they were characterised as the 13-O-β-L-fucopyranosyl-(1→2)-α-L-rhamnopyranoside 3 and 13-O-α-L-fuoopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→3)]-α-L-rhamnopyranoside 4 of the diterpene alcohol; the above assignment of the chirality at C-13 was established by measurement of nuclear Overhauser enhancement for the trisaccharide. In addition, a glycoside showing growth acceleration was also isolated and its structure was determined to be (6S,13S)-13-O-β-L-fucopyranosyl-6-O-α-L-rhamnopyranosylcleroda-3,14-diene-6,13-diol 2.

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2. (Article ID: 10871)
 
Vleggaar R, van Heerden FR, Anderson LAP, Erasmus GL
Toxic constituents of the asclepiadaceae. Structure elucidation of sarcovimiside A-C, pregnane glycosides of Sarcostemma viminale
Journal of the Chemical Society, Perkin Transactions 1 (1993) 483-487
 

The structure elucidation of three related pregnane glycosides, sarcovimiside A, B, and C from Sarcostemma viminale, is based on a detailed study of their highfield 1H and 13C NMR spectra. The results show that all the sugars are (1→4)-linked and both (β-D- and α-L-cymarose residues are present in each glycoside chain. The aglycone of sarcovimiside A was identified as 12β-benzoyloxy-3β,8β,14.17-tetrahydroxy-14β,17α-pregn-5-en-20-one whereas in the case of both sarcovimiside B and C, oxidative cleavage of the C(8)–C(14) bond occurred and the aglycone is the 12,20-O,O-dibenzoyl derivative of (20S)-3β,5,12β,17,20-pentahydroxy-8,14-seco-5β,17α-pregn-6-ene-8,14-dione. Sarcovimiside C is the β-(1→4)-glucopyranosyl derivative of sarcovimiside B.

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