Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperNCBI PubMed ID: 34245738Publication DOI: 10.1016/j.ijbiomac.2021.07.036Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Ricardo P.S. Oliveira <rpsolive
usp.br>
Institutions: Department of Biochemical and Pharmaceutical Technology, University of São Paulo, 05508-000 São Paulo, Brazil, CICECO, Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, Portugal, CEB, Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal, Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, University of Genoa, Via Opera Pia 15, 16145 Genoa, Italy
A levan-type fructooligosaccharide was produced by a Paenibacillus strain isolated from Brazilian crude oil, the purity of which was 98.5% after precipitation with ethanol and dialysis. Characterization by FTIR, NMR spectroscopy, GC-FID and ESI-MS revealed that it is a mixture of linear β(2 → 6) fructosyl polymers with average degree of polymerization (DP) of 18 and branching ratio of 20. Morphological structure and physicochemical properties were investigated to assess levan microstructure, degradation temperature and thermomechanical features. Thermal Gravimetric Analysis highlighted degradation temperature of 218 °C, Differential Scanning Calorimetry (DSC) glass transition at 81.47 °C, and Dynamic Mechanical Analysis three frequency-dependent transition peaks. These peaks, corresponding to a first thermomechanical transition event at 86.60 °C related to the DSC endothermic event, a second at 170.9 °C and a third at 185.2 °C, were attributed to different glass transition temperatures of oligo and polyfructans with different DP. Levan showed high morphological versatility and technological potential for the food, nutraceutical, and pharmaceutical industries.
levan, physicochemical characterization, thermomechanical stability
Structure type: homopolymer ; n=18
Location inside paper: p. 793
Trivial name: levan-type polysaccharide
Compound class: EPS
Contained glycoepitopes: IEDB_923066
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, PCR, X-ray, sugar analysis, ESI-MS, FTIR, RNA sequencing, GC-FID, SEM, TGA, DSC, DMA
NCBI Taxonomy refs (TaxIDs): 58172
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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