Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 33124093Publication DOI: 10.1002/anie.202013729Journal NLM ID: 0370543Publisher: Weinheim: Wiley-VCH
Correspondence: dtak
applc.keio.ac.jp; toshima
applc.keio.ac.jp
Institutions: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan
Avian pathogenic Escherichia coli (APEC) is a common bacterial pathogen infecting chickens, resulting in economic losses to the poultry industry worldwide. In particular, APEC O1, one of the most common serotypes of APEC, is considered problematic due to its zoonotic potential. Therefore, many attempts have been made to develop an effective vaccine against APEC O1. In fact, the lipopolysaccharide (LPS) O-antigen of APEC O1 has been shown to be a potent antigen for inducing specific protective immune responses. However, the detailed structure of the O-antigen of APEC O1 is not clear. The present study demonstrates the first synthesis of a pentasaccharide repeating unit of LPS derived from virulent E. coli O1 and its conjugate with BSA. ELISA tests using the semi-synthetic glycoconjugate and the APEC O1 immune chicken serum revealed that the pentasaccharide is a glycotope candidate of APEC O1, with great potential as an antigen for vaccine development.
Bacterial, Escherichia coli, glycoconjugate, avian pathogenic Escherichia coli, β-rhamnoside, boron-mediated aglycon delivery, glycotope
Structure type: polymer chemical repeating unit
Location inside paper: p. 1790, Fig. 1, O1A
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135610,IEDB_135813,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_225177,IEDB_885813,IEDB_885823
Methods: 13C NMR, TLC, ELISA, chemical synthesis, chemical methods, MALDI-TOF MS, conjugation, 1H NMr
Related record ID(s): 9708, 9709, 9710
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G57484QO, GlycomeDB:
6035
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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