Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
The structure was elucidated in this paperNCBI PubMed ID: 33667610Publication DOI: 10.1016/j.resmic.2021.103815Journal NLM ID: 8907468Publisher: Elsevier
Correspondence: johanna.kenyon
qut.edu.au
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia, M.M. Shemyakin & Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia, Department of Clinical Microbiology, Christian Medical College, Vellore, 632004, Tamil Nadu, India, Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, 66160, USA
The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy. The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar. Accordingly, biosynthesis of A. baumannii CPS types are initiated by initiating transferases (Itrs) that transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier. However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier. The itrA4 gene was found in a module transcribed in the opposite direction to the majority of the K locus. This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface. Investigation into the origins of orfKL92-gtr166-itrA4-wziKL92 revealed it might have originated from Acinetobacter junii.
capsular polysaccharide, Acinetobacter baumannii, capsule biosynthesis, galactosyl 1-phosphate transferase, initiating transferase, ItrA4
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 3, CPS
Compound class: CPS
Contained glycoepitopes: IEDB_136044,IEDB_136105,IEDB_137472,IEDB_141794,IEDB_190606,IEDB_225177,IEDB_885823,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, GLC, Smith degradation, composition analysis, HPLC, GPC, periodate oxidation, bioinformatic analysis
Biosynthesis and genetic data: Gtr1164,Gtr165, Gtr165, Gtr166, Wzy(K92)[ItrA4]
Related record ID(s): 9756, 9757
NCBI Taxonomy refs (TaxIDs): 470
Show glycosyltransferases
NMR conditions: in D2O at 333 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3,3 aLRhap 103.6 71.2 71.6 73.4 70.4 17.8-17.9
3,3,3 aLRhap 103.1 71.3-71.5 80.5 78.4 69.3 18.3
3,3 aLRhap 103.2 71.3-71.5 79.4 72.6 70.5-70.6 17.8-17.9
3 aLRhap 103.3 71.2 79.7 72.6 70.5-70.6 17.8-17.9
bDGalp 104.2 71.7 81.8 69.7 76.1 62.3
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3,3 aLRhap 5.08 4.17 3.85 3.48 3.86 1.29
3,3,3 aLRhap 5.05 4.14 4.10 3.86 3.96 1.36
3,3 aLRhap 5.06 4.14 3.92 3.59 3.88 1.30
3 aLRhap 5.08 4.17 3.92 3.59 3.88 1.29
bDGalp 4.63 3.65 3.71 4.04 3.67 3.76-3.81
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3,3 aLRhap 103.6/5.08 71.2/4.17 71.6/3.85 73.4/3.48 70.4/3.86 17.8-17.9/1.29
3,3,3 aLRhap 103.1/5.05 71.3-71.5/4.14 80.5/4.10 78.4/3.86 69.3/3.96 18.3/1.36
3,3 aLRhap 103.2/5.06 71.3-71.5/4.14 79.4/3.92 72.6/3.59 70.5-70.6/3.88 17.8-17.9/1.30
3 aLRhap 103.3/5.08 71.2/4.17 79.7/3.92 72.6/3.59 70.5-70.6/3.88 17.8-17.9/1.29
bDGalp 104.2/4.63 71.7/3.65 81.8/3.71 69.7/4.04 76.1/3.67 62.3/3.76-3.81
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3,3 | aLRhap | 5.08 | 4.17 | 3.85 | 3.48 | 3.86 | 1.29 |
| 3,3,3 | aLRhap | 5.05 | 4.14 | 4.10 | 3.86 | 3.96 | 1.36 |
| 3,3 | aLRhap | 5.06 | 4.14 | 3.92 | 3.59 | 3.88 | 1.30 |
| 3 | aLRhap | 5.08 | 4.17 | 3.92 | 3.59 | 3.88 | 1.29 |
| | bDGalp | 4.63 | 3.65 | 3.71 | 4.04 | 3.67 | 3.76
3.81 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3,3 | aLRhap | 103.6 | 71.2 | 71.6 | 73.4 | 70.4 | 17.8
17.9 |
| 3,3,3 | aLRhap | 103.1 | 71.3
71.5 | 80.5 | 78.4 | 69.3 | 18.3 |
| 3,3 | aLRhap | 103.2 | 71.3
71.5 | 79.4 | 72.6 | 70.5
70.6 | 17.8
17.9 |
| 3 | aLRhap | 103.3 | 71.2 | 79.7 | 72.6 | 70.5
70.6 | 17.8
17.9 |
| | bDGalp | 104.2 | 71.7 | 81.8 | 69.7 | 76.1 | 62.3 |
|
There is only one chemically distinct structure:
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Found 1 record.
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