Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
NCBI PubMed ID: 34070371Publication DOI: 10.3390/v13060978Journal NLM ID: 101509722Publisher: Basel, Switzerland: MDPI
Correspondence: popova_nastya86
mail.ru
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Moscow Institute of Physics and Technology (National Research University), Institutskiy per. 9, Dolgoprudny, 141700 Moscow Region, Russia, State Research Center for Applied Microbiology and Biotechnology, 24 'Quarter A' Territory, Obolensk, City District Serpukhov, 142279 Moscow Region, Russia, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia, Central Scientific Research Institute of Epidemiology, Novogireevskaya 3a, 111123 Moscow, Russia, Biology Department, Lomonosov Moscow State University, Leninskie Gory 1, 119234 Moscow, Russia, Biology Department, Shenzhen MSU-BIT University, Ruyi Rd. 299, Longgang District, Shenzhen 518172, China
Acinetobacter baumannii, one of the most significant nosocomial pathogens, is capable of producing structurally diverse capsular polysaccharides (CPSs) which are the primary receptors for A. baumannii bacteriophages encoding polysaccharide-degrading enzymes. To date, bacterial viruses specifically infecting A. baumannii strains belonging to more than ten various capsular types (K types) were isolated and characterized. In the present study, we investigate the biological properties, genomic organization, and virus-bacterial host interaction strategy of novel myovirus TaPaz isolated on the bacterial lawn of A. baumannii strain with a K47 capsular polysaccharide structure. The phage linear double-stranded DNA genome of 93,703 bp contains 178 open reading frames. Genes encoding two different tailspike depolymerases (TSDs) were identified in the phage genome. Recombinant TSDs were purified and tested against the collection of A. baumannii strains belonging to 56 different K types. One of the TSDs was demonstrated to be a specific glycosidase that cleaves the K47 CPS by the hydrolytic mechanism.
Acinetobacter baumannii, capsular polysaccharide, bacteriophage, glycosidase, capsular type, tailspike depolymerase
Structure type: polymer biological repeating unit
Location inside paper: Fig. 6, A. baumannii K47 (NIPH 601)
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_885822,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, DNA techniques, GPC, phage depolymerisation, HR-ESI-MS, TEM, phage isolation, phage infection inhibition assay, phage genome analysis
Related record ID(s): 9767, 9768
NCBI Taxonomy refs (TaxIDs): 1217637Reference(s) to other database(s): GTC:G51712EK
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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