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1. (Article ID: 1095)
Perepelov AV, Shashkov AS, Senchenkova SN, Knirel YA, Literacka E, Kaca W
Structure of the O-specific polysaccharide of the bacterium Proteus mirabilis O29
Biochemistry (Moscow) 65(2) (2000)
176-179
An O-specific polysaccharide was obtained by mild acid degradation of P. mirabilis O29 lipopolysaccharide (LPS) and found to contain 2-acetamido-2-deoxy-D-galactose and D-glucuronic acid (D-GlcA) in the ratio 3:1. Studies fo the polysaccharide by 1H- and 13C NMR spectroscopy including two-dimensional correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), nuclear Overhauser effect spectroscopy (NOESY), and H-detected 1H,13C-heteronuclear multiple-quantum coherence (HMQC) experiments demonstrated the following structure of the branched tetrasaccharide repeating unit: (see formula in text).
Lipopolysaccharide, LPS, structure, strain, structural, characterization, polysaccharide, O-antigen, bacteria, O-specific, O-specific polysaccharide, Proteus, Proteus mirabilis, serological, chemical, immunochemical
NCBI PubMed ID: 10713543Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: perepel
ioc.ac.ru
Institutions: Institute of Microbiology and Immunology, University of Lodz, Poland, Center of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland, N.D. Zelinsky Institute of Organic Chamisry, Russian Academy of Sciences, Leninsky pr. 47, Moscow, 119991 Russia
Methods: NMR-2D, NMR, sugar analysis, determination of absolute configuration
The publication contains the following compound(s):
- Compound ID: 885
|
a-D-GalpNAc-(1-3)-+
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-3)-b-D-GalpNAc-(1-4)-b-D-GalpNAc-(1-4)-b-D-GlcpA-(1- |
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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Reference(s) to other database(s): GTC:G52894IP, GlycomeDB:
27747
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