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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)

The structure was elucidated in this paper
Publication DOI: 10.1016/j.ijbiomac.2020.11.165
Journal NLM ID: 7909578
Publisher: Butterworth-Heinemann
Correspondence: Yong Liu <haoasliu

163.com>; Pin Su <supin102

163.com>
Institutions: Longping Branch, Graduate School of Hunan University, Changsha 410125, China, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China, Agricultural and Rural Bureau of Changsha County, Changsha, China, Ecological Environment Testing Center of Changsha, Changsha 410001, China

One water exopolysaccharide, designated G-EPS, was secreted by Rhodopseudomonas palustris GJ-22 culture media. The structure of G-EPS was characterized with HPGPC, GC–MS, methylation, 1D and 2D NMR, along with UV and FT-IR spectrum. The G-EPS molecular weight was 10.026 kilodalton, and is composed of D-mannose (92.8%) and d-glucose (7.2%). The purified G-EPS promoted plant growth and induced systemic resistance against TMV in Nicotiana benthamiana. These results suggested that G-EPS is an important active component of the bio-control capacity of GJ-22.

characterization, exopolysaccharide, Rhodopseudomonas palustris, Induce systemic resistance (ISR)

Structure type: structural motif or average structure ; 10026
Location inside paper: p. 166, Fig. 4, G-EPS
Trivial name: G-EPS
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141111,IEDB_141793,IEDB_141795,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_141832,IEDB_141833,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153756,IEDB_153762,IEDB_153763,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_164480,IEDB_174840,IEDB_76933,IEDB_857735,IEDB_983930,SB_136,SB_191,SB_196,SB_197,SB_198,SB_44,SB_67,SB_72,SB_77

Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, ESI-MS, acid hydrolysis, GLC, FTIR, composition analysis, HPLC, UV, RT-PCR, statistical analysis, HPGPC, plant growth promotion, TMV infection
Comments, role: one possible repeating sequence of the polysaccharide G-EPS structure; NMR temperature was not specified.

NCBI Taxonomy refs (TaxIDs): 1076
Show glycosyltransferases

NMR conditions: in D2O [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
?,6,2,2,2,3	aDManp	100.83	71.42	71.95	68.03	74.53	66.74
?,6,2,2,2	aDManp	103.54	71.55	79.30	68.40	74.75	62.16
?,6,2,2	aDManp	101.89	79.67	71.62	67.74	74.63	62.16
?,6,2,6	aDManp	103.43	70.95	71.82	67.87	74.71	62.16
?,6,2	aDManp	99.62	80.12	71.58	68.06	74.52	66.74
?,6,6	aDManp	103.43	70.95	71.82	67.87	74.71	62.16
?,6	aDManp	99.62	80.12	71.58	68.06	74.52	66.74
?	aDManp	100.83	71.42	71.95	68.03	74.53	66.74
	aDManp


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
?,6,2,2,2,3	aDManp	4.85	3.94	3.80	3.65	3.78	3.71-3.88
?,6,2,2,2	aDManp	5.09	4.03	3.95	3.60	3.78	3.69-3.82
?,6,2,2	aDManp	5.23	4.06	3.86	3.79	3.65	3.69-3.82
?,6,2,6	aDManp	5.00	4.17	3.78	3.66	3.74	3.69-3.82
?,6,2	aDManp	5.05	3.98	3.86	3.60	3.78	3.71-3.96
?,6,6	aDManp	5.00	4.17	3.78	3.66	3.74	3.69-3.82
?,6	aDManp	5.05	3.98	3.86	3.60	3.78	3.71-3.96
?	aDManp	4.85	3.94	3.80	3.65	3.78	3.71-3.88
	aDManp


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
?,6,2,2,2,3	aDManp	100.83/4.85	71.42/3.94	71.95/3.80	68.03/3.65	74.53/3.78	66.74/3.71-3.88
?,6,2,2,2	aDManp	103.54/5.09	71.55/4.03	79.30/3.95	68.40/3.60	74.75/3.78	62.16/3.69-3.82
?,6,2,2	aDManp	101.89/5.23	79.67/4.06	71.62/3.86	67.74/3.79	74.63/3.65	62.16/3.69-3.82
?,6,2,6	aDManp	103.43/5.00	70.95/4.17	71.82/3.78	67.87/3.66	74.71/3.74	62.16/3.69-3.82
?,6,2	aDManp	99.62/5.05	80.12/3.98	71.58/3.86	68.06/3.60	74.52/3.78	66.74/3.71-3.96
?,6,6	aDManp	103.43/5.00	70.95/4.17	71.82/3.78	67.87/3.66	74.71/3.74	62.16/3.69-3.82
?,6	aDManp	99.62/5.05	80.12/3.98	71.58/3.86	68.06/3.60	74.52/3.78	66.74/3.71-3.96
?	aDManp	100.83/4.85	71.42/3.94	71.95/3.80	68.03/3.65	74.53/3.78	66.74/3.71-3.88
	aDManp

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
?,6,2,2,2,3	aDManp	4.85	3.94	3.80	3.65	3.78	3.71 3.88
?,6,2,2,2	aDManp	5.09	4.03	3.95	3.60	3.78	3.69 3.82
?,6,2,2	aDManp	5.23	4.06	3.86	3.79	3.65	3.69 3.82
?,6,2,6	aDManp	5.00	4.17	3.78	3.66	3.74	3.69 3.82
?,6,2	aDManp	5.05	3.98	3.86	3.60	3.78	3.71 3.96
?,6,6	aDManp	5.00	4.17	3.78	3.66	3.74	3.69 3.82
?,6	aDManp	5.05	3.98	3.86	3.60	3.78	3.71 3.96
?	aDManp	4.85	3.94	3.80	3.65	3.78	3.71 3.88
	aDManp

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
?,6,2,2,2,3	aDManp	100.83	71.42	71.95	68.03	74.53	66.74
?,6,2,2,2	aDManp	103.54	71.55	79.30	68.40	74.75	62.16
?,6,2,2	aDManp	101.89	79.67	71.62	67.74	74.63	62.16
?,6,2,6	aDManp	103.43	70.95	71.82	67.87	74.71	62.16
?,6,2	aDManp	99.62	80.12	71.58	68.06	74.52	66.74
?,6,6	aDManp	103.43	70.95	71.82	67.87	74.71	62.16
?,6	aDManp	99.62	80.12	71.58	68.06	74.52	66.74
?	aDManp	100.83	71.42	71.95	68.03	74.53	66.74
	aDManp

There are 4 chemically distinct structures. Please, select:

[*]OC[C@H]1O[C@H](O[C@@H]2[C@H](O)[C@@H](O[C@H]3[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]3O[C@H]3[C@@H](O)[C@H](O)[C@@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)O[C@@H]3O[C@@H]3[C@@H](OC[C@H]4O[C@H](O[C@H]5[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]5[*])[C@@H](O)[C@@H](O)[C@@H]4O)O[C@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@H]2O)[C@@H](O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize