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1. (Article ID: 10990)
 
Sadeh S, Warren CD, Daniel PF, Bugge B, James LF, Jeanloz RW
Characterization of oligosaccharides from the urine of loco-intoxicated sheep
FEBS Letters 163 (1983) 104-109
 

Two major oligosaccharides were isolated by preparative HPLC from the urine of a locoweed-fed sheep. Analysis by gas-liquid chromatography and mass-spectrometry indicated compositions of (Man)4(GlcNAc)2 and (Man)5(GlcNAc)2, respectively. Structures were determined by digestion with α-D-mannosidase and endo-β-N-acetylglucosaminidases D and H, and comparison of the products by HPLC with synthetic standards, and oligosaccharides isolated from human mannosidosis urine. Incubation with an exo-β-N-acetylglucosaminidase was without effect.

oligosaccharide, structure, sheep locoweed, urine

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