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1. (Article ID: 1102)
Perry MB, MacLean LL
Structural characterization of the antigenic O-chain of the lipopolysaccharide of Escherichia coli serotype O65
Carbohydrate Research 322(1-2) (1999)
57-66
The antigenic O-polysaccharide moiety of the lipopolysaccharide produced by Escherichia coli serotype O65 was investigated by composition, methylation, base hydrolysis, periodate oxidation, mass spectrometric methods, and by 1D and 2D NMR spectroscopy. The O-polysaccharide had [a]D108° (water) and is a high-molecular-weight unbranched linear polymer of repeating pentasaccharide units composed of 1:1:1:1:1 D-galacturonic acid (D-GalA), D-galacturonamide (D-GalANH2), 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), 2-acetamido-2-deoxy-D-galactose (DGalNAc),and 3-acetamido-3,6-dideoxy-D-glucose (D-Qui3NAc), and has the following structure: [see formula in text]
Lipopolysaccharide, Escherichia coli, NMR spectroscopy, polysaccharide structure
NCBI PubMed ID: 10629949Publication DOI: 10.1016/S0008-6215(99)00212-8Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: malcolm.perry
nrc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ont., Canada K 1A 0R 6
Methods: methylation, periodate oxidation, NMR-2D, NMR, MS, composition analysis, base hydrolysis
The publication contains the following compound(s):
- Compound ID: 3048
|
-2)-b-D-Quip3NAc-(1-4)-a-D-GalpA6NH2-(1-4)-a-D-GalpNAc-(1-4)-b-D-GalpA-(1-3)-a-D-GlcpNAc-(1- |
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Structure type: suggested polymer biological repeating unit
Compound class: O-polysaccharide, O-antigen
Reference(s) to other database(s): GTC:G86652HR, GlycomeDB:
28132
- Compound ID: 3049
|
b-L-4dthrHexp4enA-(1-4)-a-D-GalpNAc-(1-4)-b-D-GalpA-(1-3)-a-D-GlcpNAc-(1-2)-D-Qui3NAc-ol |
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Structure type: oligomer
Reference(s) to other database(s): GlycomeDB:
36771
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