The structures of two cell wall teichoic acids of Brevibacterium iodinum VKM Ac-2106(T) were studied. The structure of mannitol teichoic acid described earlier was mainly confirmed. This polymer is 1,6-poly(mannitol phosphate) bearing β-D-glucopyranosyl residues at the C-2 of mannitol and pyruvic acid residues at the C-4 and C-5. The absolute configurations of D-mannitol and S-pyruvic acid were found. The following distinctions from the earlier described structure were found: unsubstituted 1,6-poly(mannitol phosphate) residues and residues substituted only by β-D-glucopyranosyl at the C-2 of mannitol but unsubstituted by pyruvic acid are present in the chain. The structure of glycerol teichoic acid present in the cell wall as a minor component (~7%) is also described. This acid is identified as 1,3-poly(glycerol phosphate) substituted at the C-2 of glycerol by 2-acetamido-2-deoxy-α-D-galactopyranosyl residues bearing R-pyruvic acid residues at the C-4 and C-6 of galactose. This polymer is for the first time described in the cell wall of Gram-positive bacteria.
teichoic acids, Brevibacterium, poly(glycerol phosphate), Pyruvic acid acetal, NMR-spectroscopy, poly(mannitol phosphate)
NCBI PubMed ID: 15627390Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: potekchina@hotbox.ru
Institutions: Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia
Methods: 13C NMR, 1H NMR, HF solvolysis, sugar analysis, acid hydrolysis, paper chromatography, paper electrophoresis, 32P NMR
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