Further studies by means of preparative HPLC led to the isolation of two new furostanol saponins, macrostemonoside J (1) and L (3), along with an artifact, macrostemonoside K (2) from the bulbs of Allium macrostemon. On the basis of chemical evidence and spectral analysis (^1H, ^<13>C-NMR and FAB-MS), the structure of 1 was elucidated to be 26-O-β-D-glucopyranosyl 2β,3β,22,26-tetrahydroxy-25(R)-5β-furostan 3-O-β-D-glucopyranosyl(1→2)-β-D-galactopyranoside. 3 was deduced to be 26-O-β-D-glucopyranosyl 2β,3β,26-trihydroxy-25(R)-5β-furostan-20(22)-ene 3-O-β-D-glucopyranosyl(1→2)-β-D-galactopyranoside.

furostanol saponin, Allium macrostemon, macrostemonoside J, macrostemonoside L

• Compound ID: 27483
  

  b-D-Glcp-(1-26)-+ | b-D-Glcp-(1-2)-b-D-Galp-(1-3)-Subst Subst = 25R-5β-furostan-2β,3β,22,26-tetrol = SMILES [H][C@]1(O{22}C(CC[C@@H](C){26}CO)(O)[C@H]2C)C[C@@]3([H])[C@]4([H])CC[C@]5([H])C{3}[C@@H](O){2}[C@@H](O)C[C@]5(C)[C@@]4([H])CC[C@]3(C)[C@]12[H]

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  Structure type: oligomer ; 919 [M+H-H2O]+
  Trivial name: macrostemonoside J
  Compound class: saponin glycoside
  Reference(s) to other database(s): CCSD:8786, CBank-STR:17087
  
   
  
  Allium macrostemon
  CSDB ID 120509 (all data & tools)
• Compound ID: 27484
  

  b-D-Glcp-(1-26)-+ | b-D-Glcp-(1-2)-b-D-Galp-(1-3)-Subst22Me Subst = 25R-5β-furostan-2β,3β,22,26-tetrol = SMILES [H][C@]1(O{22}C(CC[C@@H](C){26}CO)(O)[C@H]2C)C[C@@]3([H])[C@]4([H])CC[C@]5([H])C{3}[C@@H](O){2}[C@@H](O)C[C@]5(C)[C@@]4([H])CC[C@]3(C)[C@]12[H]

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  Structure type: oligomer ; 949 [M-H]-
  Trivial name: macrostemonoside K
  Compound class: saponin glycoside
  
   
  
  Allium macrostemon
  CSDB ID 220509 (all data & tools)
• Compound ID: 27485
  

  b-D-Glcp-(1-26)-+ | b-D-Glcp-(1-2)-b-D-Galp-(1-3)-Subst Subst = 25R-5β-furost-20(22)-en-2β,3β,26-triol = SMILES [H][C@]1(OC(CC[C@@H](C){26}CO)=C2C)C[C@@]3([H])[C@]4([H])CC[C@]5([H])C{3}[C@@H](O){2}[C@@H](O)C[C@]5(C)[C@@]4([H])CC[C@]3(C)[C@]12[H]

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  Structure type: oligomer ; 941 [M+Na]+
  Trivial name: macrostemonoside L
  Compound class: saponin glycoside
  
   
  
  Allium macrostemon
  CSDB ID 270509 (all data & tools)