Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperNCBI PubMed ID: 25798529Publication DOI: 10.1021/acs.jafc.5b01086Journal NLM ID: 0374755Publisher: American Chemical Society
Correspondence: dongms
njau.edu.cn
Institutions: aggerInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210095, P. R. China, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P. R. China
A novel cell-bound exopolysaccharide (c-EPS) was isolated from Lactobacillus helveticus MB2-1 by ultrasonic extraction, anion exchange, and gel filtration chromatography before being structurally characterized. The c-EPS is a heteropolysaccharide with an average molecular weight of 1.83 x 10(5) Da and is composed of glucose, mannose, galactose, rhamnose, and arabinose at a molar ratio of 3.12:1.01:1.00:0.18:0.16. Methylation analysis and nuclear magnetic resonance analysis revealed that the c-EPS is a linear glucomannogalactan containing repeating units of →6)-β-D-Manp-(1→3)-β-D-Glcp-(1→3)-β-D-Glcp-(1→3)-β-D-Glcp-(1→4)-α-D-Galp-(1→ and trace amounts of Rhap-(1→ and (1→4)-Arap residues. Complex formation with Congo red demonstrated a triple-strand helical conformation for the c-EPS. Scanning electron microscopy of the c-EPS revealed many regular feather-like structural units. Topographical examination of c-EPS by atomic force microscopy revealed that the c-EPS formed rounded-to-spherical lumps with different sizes and chain formations. Furthermore, preliminary in vitro tests revealed that c-EPS significantly inhibited the proliferation of HepG-2, BGC-823, and especially HT-29 cancer cells.
anticancer activity, Structural characterization, Lactobacillus helveticus MB2-1, cell-bound exopolysaccharide (c-EPS)
Structure type: polymer chemical repeating unit ; 183000, n=~231
Location inside paper: abstract, fig.5, c-EPS
Trivial name: c-EPS
Compound class: EPS
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_137485,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153543,IEDB_158555,IEDB_190606,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_7,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, sugar analysis, acid hydrolysis, GC, NMR-1D, HPSEC, UV, atomic force microscopy, statistical analysis, DEAE, lectron microscopy
Biological activity: the in vitro anticancer activity of c-EPS against human HepG-2 liver cancer, BGC-823 gastric cancer, and HT-29 colon cancer cells were evaluated
NCBI Taxonomy refs (TaxIDs): 1587Reference(s) to other database(s): GTC:G47647ZF
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There is only one chemically distinct structure:
Found 
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