The sugar coat of 'Black Death' betrays it. The plague can be detected by monoclonal anti-carbohydrate antibodies. In a new technique, a plague-specific oligosaccharide antigen (see structure) is synthesized. With the help of lipopolysaccharide (LPS)-specific monoclonal antibodies (mAbs), the presence of the plague pathogen Yersinia pestis can then be detected in serum from patients by using a glycan microarray.
lipopolysaccharides, antibodies, Oligosaccharides, Yersinia pestis, pathogen detection
Publication DOI: 10.1002/anie.201301633Journal NLM ID: 0370543Publisher: Weinheim: Wiley-VCH
Correspondence: M. Caroff
; peter.seeberger@mpikg.mpg.de
Institutions: Prof. Dr. P. H. Seeberger Institute of Chemistry and Biochemistry Freie Universitt Berlin Arnimallee 22, 14195 Berlin, Germany
Methods: 13C NMR, 1H NMR, SDS-PAGE, chemical methods, MALDI-TOF MS, serological methods, conjugation
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