O-polysaccharides were isolated from lipopolysaccharides obtained from four different strains of plant pathogenic bacteria belonging to the species Dickeya solani: two of them were isolated in Poland (IFB0099 and IFB0158), the third in Germany (IFB0223) and the last one, D. solani Type Strain IPO2222, originated from the Netherlands. In addition, the O-polysaccharide of a closely related species D. dadantii strain 3937 was isolated. The purified polysaccharides of the five strains were analyzed using NMR spectroscopy and chemical methods. Sugar and methylation analyses, including absolute configuration assignment, together with NMR data revealed that all O-polysaccharides tested are homopolymers of 6-deoxy-d-altrose (d-6dAlt) the following structure: →2)-β-d-6dAltp-(1→.
Lipopolysaccharide, NMR, structure, O-polysaccharide, NMR-spectroscopy, 6-deoxy-D-altrose, Dickeya dadantii, Dickeya solani
NCBI PubMed ID: 28395253Publication DOI: 10.1016/j.carres.2017.04.001Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: zbigniew.kaczynski@ug.edu.pl
Institutions: Faculty of Chemistry, University of Gdansk, Gdansk, Poland, Univ Lyon, Universite Lyon 1, INSA de Lyon, CNRS UMR5240 Microbiologie Adaptation et Pathogenie, F-69622, Villeurbanne, France, Department of Biotechnology, Faculty of Biotechnology University of Gdansk, Medical University of Gdansk, Abrahama 58, 80-307, Gdansk, Poland
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, acid hydrolysis, GLC, GPC
Found