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Yeh KM, Chiu SK, Lin CL, Huang LY, Tsai YK, Chang JC, Lin JC, Chang FY, Siu LK
Surface antigens contribute differently to the pathophysiological features in serotype K1 and K2 Klebsiella pneumoniae strains isolated from liver abscesses
Gut Pathogens 8 (2016)
4
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S-Pyr-(2-3:2-2)-+
|
-4)-b-D-GlcpA-(1-4)-a-L-Fucp-(1-3)-b-D-Glcp-(1- |
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Klebsiella pneumoniae K1
(Ancestor NCBI TaxID 573,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Klebsiella pneumoniae [ICD11:
XN741 
]
NCBI PubMed ID: 26893615Publication DOI: 10.1186/s13099-016-0085-5Journal NLM ID: 101474263Publisher: London: BioMed Central
Correspondence: fychang
ndmctsgh.edu.tw; lksiu
nhri.org.tw
Institutions: Infection Control Office, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Cheng-Kung Road, Neihu, 114 Taipei City Taiwan, School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan, Department of Internal Medicine, Division of Pulmonary Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan, Department of Internal Medicine, Division of Infectious Diseases and Tropical Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Cheng-Kung Road, Neihu, 114 Taipei City Taiwan, Institute of Infectious Diseases and Vaccine Research, National Health Research Institutes, 35 Keyan Road, Zhunan, 35053 Miaoli, Taiwan, Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan
BACKGROUND: The virulence role of surface antigens in a single serotype of Klebsiella pneumoniae strain have been studied, but little is known about whether their contribution will vary with serotype. METHOD: To investigate the role of K and O antigen in hyper-virulent strains, we constructed O and K antigen deficient mutants from serotype K1 STL43 and K2 TSGH strains from patients with liver abscess, and characterized their virulence in according to the abscess formation and resistance to neutrophil phagocytosis, serum, and bacterial clearance in liver. RESULTS: Both of K1 and K2-antigen mutants lost their wildtype resistance to neutrophil phagocytosis and hepatic clearance, and failed to cause abscess formation. K2-antigen mutant became serum susceptible while K1-antigen mutant maintained its resistance to serum killing. The amount of glucuronic acid, indicating the amount of capsular polysaccharide (CPS, K antigen), was inversed proportional to the rate of phagocytosis. O-antigen mutant of serotype K1 strains had significantly more amount of CPS, and more resistant to neutrophil phagocytosis than its wildtype counterpart. O-antigen mutants of serotype K1 and K2 strains lost their wildtype serum resistance, and kept resistant to neutrophil phagocytosis. While both mutants lacked the same O1 antigen, O-antigen mutant of serotype K1 became susceptible to liver clearance and cause mild abscess formation, but its serotype K2 counterpart maintained these wildtype virulence. CONCLUSION: We conclude that the contribution of surface antigens to virulence of K. pneumoniae strains varies with serotypes.
virulence, K-antigen, serotype, O-antigen, capsular polysaccharide, Klebsiella pneumoniae, vaccine, phagocytosis, surface antigen
Structure type: polymer chemical repeating unit
Location inside paper: p.5
Trivial name: K-antigen, S-53 EPS
Compound class: CPS, EPS
Contained glycoepitopes: IEDB_115136,IEDB_136045,IEDB_140630,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_146664,IEDB_152214,IEDB_174333,IEDB_423153,IEDB_983931,SB_192,SB_86
Methods: PCR, Western blotting, serological methods, genetic methods, serum bactericidal assays, statistical analysis, human neutrophil phagocytosis assay
Related record ID(s): 11621, 11622, 11623
NCBI Taxonomy refs (TaxIDs): 573
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There is only one chemically distinct structure:
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Yeh KM, Chiu SK, Lin CL, Huang LY, Tsai YK, Chang JC, Lin JC, Chang FY, Siu LK
Surface antigens contribute differently to the pathophysiological features in serotype K1 and K2 Klebsiella pneumoniae strains isolated from liver abscesses
Gut Pathogens 8 (2016)
4
|
a-D-GlcpA-(1-3)-+
|
-3)-b-D-Glcp-(1-4)-b-D-Manp-(1-4)-a-D-Glcp-(1- |
Show graphically |
Klebsiella pneumoniae K2
(Ancestor NCBI TaxID 573,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
NCBI PubMed ID: 26893615Publication DOI: 10.1186/s13099-016-0085-5Journal NLM ID: 101474263Publisher: London: BioMed Central
Correspondence: fychang
ndmctsgh.edu.tw; lksiu
nhri.org.tw
Institutions: Infection Control Office, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Cheng-Kung Road, Neihu, 114 Taipei City Taiwan, School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan, Department of Internal Medicine, Division of Pulmonary Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan, Department of Internal Medicine, Division of Infectious Diseases and Tropical Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Cheng-Kung Road, Neihu, 114 Taipei City Taiwan, Institute of Infectious Diseases and Vaccine Research, National Health Research Institutes, 35 Keyan Road, Zhunan, 35053 Miaoli, Taiwan, Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan
BACKGROUND: The virulence role of surface antigens in a single serotype of Klebsiella pneumoniae strain have been studied, but little is known about whether their contribution will vary with serotype. METHOD: To investigate the role of K and O antigen in hyper-virulent strains, we constructed O and K antigen deficient mutants from serotype K1 STL43 and K2 TSGH strains from patients with liver abscess, and characterized their virulence in according to the abscess formation and resistance to neutrophil phagocytosis, serum, and bacterial clearance in liver. RESULTS: Both of K1 and K2-antigen mutants lost their wildtype resistance to neutrophil phagocytosis and hepatic clearance, and failed to cause abscess formation. K2-antigen mutant became serum susceptible while K1-antigen mutant maintained its resistance to serum killing. The amount of glucuronic acid, indicating the amount of capsular polysaccharide (CPS, K antigen), was inversed proportional to the rate of phagocytosis. O-antigen mutant of serotype K1 strains had significantly more amount of CPS, and more resistant to neutrophil phagocytosis than its wildtype counterpart. O-antigen mutants of serotype K1 and K2 strains lost their wildtype serum resistance, and kept resistant to neutrophil phagocytosis. While both mutants lacked the same O1 antigen, O-antigen mutant of serotype K1 became susceptible to liver clearance and cause mild abscess formation, but its serotype K2 counterpart maintained these wildtype virulence. CONCLUSION: We conclude that the contribution of surface antigens to virulence of K. pneumoniae strains varies with serotypes.
virulence, K-antigen, serotype, O-antigen, capsular polysaccharide, Klebsiella pneumoniae, vaccine, phagocytosis, surface antigen
Structure type: polymer chemical repeating unit
Location inside paper: p.5
Trivial name: K-antigen
Compound class: CPS, O-polysaccharide
Contained glycoepitopes: IEDB_115136,IEDB_1334432,IEDB_1334433,IEDB_1334434,IEDB_1334435,IEDB_137485,IEDB_140630,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_153755,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72
Methods: PCR, Western blotting, serological methods, genetic methods, serum bactericidal assays, statistical analysis, human neutrophil phagocytosis assay
Comments, role: published polymerization frame was shifted for conformity with other records.
Related record ID(s): 11260, 11622, 11623
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G73682RA, GlycomeDB:
2875
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There is only one chemically distinct structure:
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Yeh KM, Chiu SK, Lin CL, Huang LY, Tsai YK, Chang JC, Lin JC, Chang FY, Siu LK
Surface antigens contribute differently to the pathophysiological features in serotype K1 and K2 Klebsiella pneumoniae strains isolated from liver abscesses
Gut Pathogens 8 (2016)
4
Klebsiella pneumoniae O1
(Ancestor NCBI TaxID 573,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
NCBI PubMed ID: 26893615Publication DOI: 10.1186/s13099-016-0085-5Journal NLM ID: 101474263Publisher: London: BioMed Central
Correspondence: fychang
ndmctsgh.edu.tw; lksiu
nhri.org.tw
Institutions: Infection Control Office, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Cheng-Kung Road, Neihu, 114 Taipei City Taiwan, School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan, Department of Internal Medicine, Division of Pulmonary Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan, Department of Internal Medicine, Division of Infectious Diseases and Tropical Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Cheng-Kung Road, Neihu, 114 Taipei City Taiwan, Institute of Infectious Diseases and Vaccine Research, National Health Research Institutes, 35 Keyan Road, Zhunan, 35053 Miaoli, Taiwan, Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan
BACKGROUND: The virulence role of surface antigens in a single serotype of Klebsiella pneumoniae strain have been studied, but little is known about whether their contribution will vary with serotype. METHOD: To investigate the role of K and O antigen in hyper-virulent strains, we constructed O and K antigen deficient mutants from serotype K1 STL43 and K2 TSGH strains from patients with liver abscess, and characterized their virulence in according to the abscess formation and resistance to neutrophil phagocytosis, serum, and bacterial clearance in liver. RESULTS: Both of K1 and K2-antigen mutants lost their wildtype resistance to neutrophil phagocytosis and hepatic clearance, and failed to cause abscess formation. K2-antigen mutant became serum susceptible while K1-antigen mutant maintained its resistance to serum killing. The amount of glucuronic acid, indicating the amount of capsular polysaccharide (CPS, K antigen), was inversed proportional to the rate of phagocytosis. O-antigen mutant of serotype K1 strains had significantly more amount of CPS, and more resistant to neutrophil phagocytosis than its wildtype counterpart. O-antigen mutants of serotype K1 and K2 strains lost their wildtype serum resistance, and kept resistant to neutrophil phagocytosis. While both mutants lacked the same O1 antigen, O-antigen mutant of serotype K1 became susceptible to liver clearance and cause mild abscess formation, but its serotype K2 counterpart maintained these wildtype virulence. CONCLUSION: We conclude that the contribution of surface antigens to virulence of K. pneumoniae strains varies with serotypes.
virulence, K-antigen, serotype, O-antigen, capsular polysaccharide, Klebsiella pneumoniae, vaccine, phagocytosis, surface antigen
Structure type: polymer chemical repeating unit
Location inside paper: p. 5, D-galactan I
Trivial name: D-galactan I, galactan I, galactan, D-galactan, microbial carbohydrate determinants, D-galactan-I
Compound class: CPS, EPS, O-polysaccharide, O-antigen, LPS, cell wall polysaccharide
Contained glycoepitopes: IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,IEDB_2229966,SB_7
Methods: PCR, Western blotting, serological methods, genetic methods, serum bactericidal assays, statistical analysis, human neutrophil phagocytosis assay
Related record ID(s): 11260, 11621, 11623
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G85324FU, GlycomeDB:
665
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There is only one chemically distinct structure:
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Yeh KM, Chiu SK, Lin CL, Huang LY, Tsai YK, Chang JC, Lin JC, Chang FY, Siu LK
Surface antigens contribute differently to the pathophysiological features in serotype K1 and K2 Klebsiella pneumoniae strains isolated from liver abscesses
Gut Pathogens 8 (2016)
4
Klebsiella pneumoniae O1
(Ancestor NCBI TaxID 573,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
NCBI PubMed ID: 26893615Publication DOI: 10.1186/s13099-016-0085-5Journal NLM ID: 101474263Publisher: London: BioMed Central
Correspondence: fychang
ndmctsgh.edu.tw; lksiu
nhri.org.tw
Institutions: Infection Control Office, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Cheng-Kung Road, Neihu, 114 Taipei City Taiwan, School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan, Department of Internal Medicine, Division of Pulmonary Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan, Department of Internal Medicine, Division of Infectious Diseases and Tropical Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Cheng-Kung Road, Neihu, 114 Taipei City Taiwan, Institute of Infectious Diseases and Vaccine Research, National Health Research Institutes, 35 Keyan Road, Zhunan, 35053 Miaoli, Taiwan, Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan
BACKGROUND: The virulence role of surface antigens in a single serotype of Klebsiella pneumoniae strain have been studied, but little is known about whether their contribution will vary with serotype. METHOD: To investigate the role of K and O antigen in hyper-virulent strains, we constructed O and K antigen deficient mutants from serotype K1 STL43 and K2 TSGH strains from patients with liver abscess, and characterized their virulence in according to the abscess formation and resistance to neutrophil phagocytosis, serum, and bacterial clearance in liver. RESULTS: Both of K1 and K2-antigen mutants lost their wildtype resistance to neutrophil phagocytosis and hepatic clearance, and failed to cause abscess formation. K2-antigen mutant became serum susceptible while K1-antigen mutant maintained its resistance to serum killing. The amount of glucuronic acid, indicating the amount of capsular polysaccharide (CPS, K antigen), was inversed proportional to the rate of phagocytosis. O-antigen mutant of serotype K1 strains had significantly more amount of CPS, and more resistant to neutrophil phagocytosis than its wildtype counterpart. O-antigen mutants of serotype K1 and K2 strains lost their wildtype serum resistance, and kept resistant to neutrophil phagocytosis. While both mutants lacked the same O1 antigen, O-antigen mutant of serotype K1 became susceptible to liver clearance and cause mild abscess formation, but its serotype K2 counterpart maintained these wildtype virulence. CONCLUSION: We conclude that the contribution of surface antigens to virulence of K. pneumoniae strains varies with serotypes.
virulence, K-antigen, serotype, O-antigen, capsular polysaccharide, Klebsiella pneumoniae, vaccine, phagocytosis, surface antigen
Structure type: polymer chemical repeating unit
Location inside paper: p. 5, D-galactan II
Trivial name: D-galactan II, galactan II, D-galactan-II
Compound class: CPS, EPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_149558,IEDB_151528,IEDB_190606,IEDB_2229966,IEDB_918314,SB_165,SB_166,SB_187,SB_195,SB_7,SB_87,SB_88
Methods: PCR, Western blotting, serological methods, genetic methods, serum bactericidal assays, statistical analysis, human neutrophil phagocytosis assay
Comments, role: published polymerization frame was shifted for conformity with other records.
Related record ID(s): 11260, 11621, 11622
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G16623PZ, GlycomeDB:
664
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There is only one chemically distinct structure:
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