Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 26963608Publication DOI: 10.1016/j.carres.2016.02.007Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: fenglu63
nankai.edu.cn
Institutions: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin, China, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prosp., 47, Moscow, Russia
The wbaD gene and wbaC gene from Escherichia coli O77 O-antigen gene cluster encoding mannosyltransferases were functionally characterized in vitro. A synthetic acceptor P(1)-(11-phenoxyundecyl)-P(2)-(2-acetamido-2-deoxy-α-D-glucopyranosyl) diphosphate (GlcNAc-PP-PhU) was used as an acceptor and GDP-Man as a donor substrate; the activities of WbaD and WbaC were confirmed by detailed structural characterization of their lipooligosacharide enzyme products using high-sensitivity negative-ion electrospray ionization (ESI) collision-induced dissociation tandem mass spectrometry (CID) MS-MS. The extensive fragmentation unequivocally demonstrated that the Man(1-3)-GlcNAc linkage in WbaD catalyzed reaction product and two Man(1-2)-Man linkages in tandem WbaD/WbaC catalyzed reaction product are present, respectively. This study provided valuable information for the understanding of diversified glycosyltransferase (GT) functions and the two GTs characterized can serve as additional enzyme sources for possible pharmaceutical related applications.
O-antigen, Escherichia coli, O-antigen repeating unit, glycosyltransferases, E.coli O77, Electrospray ionization multistage tandem mass spectrometry, WbaC, WbaD
Structure type: suggested polymer biological repeating unit
Location inside paper: p.25, fig.1
Compound class: O-polysaccharide, core oligosaccharide, O-antigen
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141807,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_151531,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: ESI-MS, ESI-MS/MS, genetic methods, biochemical methods, enzyme assay, ESI-CID-MS
Biosynthesis and genetic data: genetic data
Related record ID(s): 5925, 20665, 21421, 30340
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G87308MU, GlycomeDB:
2505
Show glycosyltransferases
There is only one chemically distinct structure:
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