Susceptible and resistant cotton lines were cytologically and histochemically investigated for their defense reactions to a highly aggressive and defoliating strain of Verticillium dahliae, a fungus responsible for vascular wilt. Cytochemistry showed that early responses consisted of reinforcement in structural barriers with polysaccharides, including callose and cellulose. Ultrastructural modifications of parenchyma cells of the vascular tissues were associated with strong production of terpenoids and phenolics. These defense reactions were detected early in roots of the resistant line, one to four days after inoculation, while they were seen later in roots of the susceptible line.

ultrastructure, terpenoids, phenolics, callose, cytochemistry, vascular wilt

• Compound ID: 4415
  

  -3)-b-D-Glcp-(1-

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  Structure type: homopolymer
  Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
  Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
  Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:157, CCSD:50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
  
   
  
  Gossypium hirsutum, Gossypium barbadense
  CSDB ID 40047 (all data & tools)
• Compound ID: 6650
  

  -4)-b-D-Glcp-(1-

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  Structure type: homopolymer
  Trivial name: cellulose, β-(1,4)-glucan, cellulose, β-(1,4)-glucan
  Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
  Reference(s) to other database(s): GTC:G75830OM, GlycomeDB:27885, CCSD:50051, CBank-STR:4229
  
   
  
  Gossypium hirsutum, Gossypium barbadense
  CSDB ID 40042 (all data & tools)

Six new triterpenoids, 3β,23,24-trihydroxyolean-12-en-28-oic acid (1), 3β,6β,24-trihydroxyolean-12-en-28-oic acid (2), 3β,6β,19α,24-tetrahydroxyurs-12-en-28-oic acid (3), cincholic acid 3β-O-β-d-fucopyranoside (4), pyrocincholic acid 3β,-O-β-d-fucopyranoside (5), and pyrocincholic acid 3β-O-α-l-rhamnopyranoside (6) were isolated from the roots of Adina rubella. Their structures were elucidated by spectral analysis. The 13C NMR signals of 5 and 6 were assigned by 2D-NMR experiments.

triterpenoid, Adina rubella, pyrocincholic acid glycosides

• Compound ID: 29942
  

  b-D-Fucp-(1-3)-Subst Subst = cincholic acid = SMILES C[C@]12CC{3}[C@@H](C([C@@H]1CC[C@@]3([C@@H]2CC=C4[C@]3(CC[C@@]5([C@H]4CC(CC5)(C)C){28}C(=O)O){27}C(=O)O)C)(C)C)O

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  Structure type: monomer ; 655 [M+Na]
  C36H56O9
  Compound class: saponin glycoside
  
   
  
  Adina rubella
  CSDB ID 62358 (all data & tools)
• Compound ID: 29943
  

  b-D-Fucp-(1-3)-Subst Subst = pyrocincholic acid = SMILES C[C@]12CC{3}[C@@H](C([C@@H]1CC[C@@]3([C@@H]2CCC4=C3CC[C@@]5([C@H]4CC(CC5)(C)C){28}C(=O)O)C)(C)C)O

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  Structure type: monomer ; 611 [M+Na]
  C35H56O7
  Compound class: saponin glycoside
  
   
  
  Adina rubella
  CSDB ID 62359 (all data & tools)
• Compound ID: 29944
  

  a-L-Rhap-(1-3)-Subst Subst = pyrocincholic acid = SMILES C[C@]12CC{3}[C@@H](C([C@@H]1CC[C@@]3([C@@H]2CCC4=C3CC[C@@]5([C@H]4CC(CC5)(C)C){28}C(=O)O)C)(C)C)O

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  Structure type: monomer ; 611 [M+Na]
  C35H56O7
  Compound class: saponin glycoside
  
   
  
  Adina rubella
  CSDB ID 62360 (all data & tools)
• Compound ID: 29945
  

  b-D-Quip-(1-3)-+ | b-D-Glcp-(1-28)-Subst Subst = pyrocincholic acid = SMILES C[C@]12CC{3}[C@@H](C([C@@H]1CC[C@@]3([C@@H]2CCC4=C3CC[C@@]5([C@H]4CC(CC5)(C)C){28}C(=O)O)C)(C)C)O

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  Structure type: oligomer
  Compound class: saponin glycoside
  
   
  
  Isertia haenkeana
  CSDB ID 62361 (all data & tools)